Qualité du mélange dans un bioréacteur à cuve agitée utilisé pour la culture de cellules animales: étude de l’hétérogénéité à l’échelle laboratoire et prédiction de l’évolution du temps de mélange avec le scale-up

Abstract

Animal cells are industrially cultivated inside stirred bioreactors to produce proteinic compounds. Due to the use of mild agitation conditions in order to limit mechanical constraints, the homogeneity of the culture medium can be far from perfect. This study has therefore two objectives. 1) the global characterization of the mixing via the mixing time 2) the local description of concentration fields. The mixing time is measured by conductimetry inside 20 L, 80 L, 600L tanks. The Grenville correlation is adjusted on these experimental measurements to improve the prediction of the mixing time during the scale-up of the process. The concentration fields are visualized by the Planar Laser Induced Fluorescence (P.L.I.F) technique in the 20 L tank. This part of the study is focused on the time evolution of the maximum value of the tracer concentration inside measurement planes and of the numerical distribution of theses concentration fields.Développement d'un modèle hydrodynamique multi-échelle décrivant le mélange dans les bioréacteurs utilisés en culture cellulair