Cheng-Chih Tsai

Chun-Chih Huang

Hui-Ching Wu

Pei-Pei Lin

You-Miin Hsieh

Zi-yi Zhang

The Scientific World Journal

This study collected different probiotic isolates from animal and plant sources to evaluate the bile-salt hydrolase activity of probioticsin vitro. The deconjugation potential of bile acid was determined using high-performance liquid chromatography. HepG2 cells were cultured with probiotic strains with high BSH activity. The triglyceride (TG) and apolipoprotein B (apo B) secretion by HepG2 cells were evaluated. Our results show that the BSH activity and bile-acid deconjugation abilities ofPediococcus acidilacticiNBHK002,Bifidobacterium adolescentisNBHK006,Lactobacillus rhamnosusNBHK007, andLactobacillus acidophilusNBHK008 were higher than those of the other probiotic strains. The cholesterol concentration in cholesterol micelles was reduced within 24 h. NBHK007 reduced the TG secretion by 100% after 48 h of incubation. NBHK002, NBHK006, and NBHK007 could reduce apo B secretion by 33%, 38%, and 39%, respectively, after 24 h of incubation. The product PROBIO S-23 produced a greater decrease in the total concentration of cholesterol, low-density lipoprotein, TG, and thiobarbituric acid reactive substance in the serum or livers of hamsters with hypercholesterolemia compared with that of hamsters fed with a high-fat and high-cholesterol diet. These results show that the three probiotic strains of lactic acid bacteria are better candidates for reducing the risk of cardiovascular disease.</jats:p

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The Scientific World JOURNAL

Cholesterol-Lowering Potentials of Lactic Acid Bacteria Based on Bile-Salt Hydrolase Activity and Effect of Potent Strains on Cholesterol Metabolism<i>In Vitro</i>and<i>In Vivo</i>

http://downloads.hindawi.com/journals/tswj/2014/690752.pdf

Cholesterol-Lowering Potentials of Lactic Acid Bacteria Based on Bile-Salt Hydrolase Activity and Effect of Potent Strains on Cholesterol Metabolism
                    In Vitro

Cholesterol-Lowering Potentials of Lactic Acid Bacteria Based on Bile-Salt Hydrolase Activity and Effect of Potent Strains on Cholesterol Metabolism In Vitro

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