Adriana Pardini Vicentini Moreira

Bialek R

Cidia Vasconcellos

Freitas RS

Fugita S

Gil Benard

Katia Cristina Dantas

Kauffman CA

Marcos Vinicius da Silva

Maubon D

Paulo Ricardo Criado

Roseli S. Freitas

Wheat J

Wheat LJ

Anais Brasileiros de Dermatologia

The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8). The PCR sequences HC18, HC100 and HC5.8 resulted in a specificity of 100%. The molecular assays may increase the specificity, sensitivity and speed in the diagnosis of Histoplasmosis.</jats:p

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The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients*

http://www.scielo.br/pdf/abd/v88n1/0365-0596-abd-88-1-0141.pdf

The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients*

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