Adamala

Bayley

Beales

Carreras

Casadevall

Deshpande

Elani

Friddin

Funakoshi

Gardner

Gerber

Huang

Hwang

Karamdad

Karzbrun

Kurihara

Lentini

Luisi

Martino

Matosevic

Niederholtmeyer

Noireaux

Shin

Shum

Squires

Stano

Szita

Szostak

Takinoue

Tang

Theberge

van Meer

van Swaay

Villar

Yamada

Yuval Elani

Zhang

The quest to construct artificial cells from the bottom-up using simple building blocks has received much attention over recent decades and is one of the grand challenges in synthetic biology. Cell mimics that are encapsulated by lipid membranes are a particularly powerful class of artificial cells due to their biocompatibility and the ability to reconstitute biological machinery within them. One of the key obstacles in the field centres on the following: how can membrane-based artificial cells be generated in a controlled way and in high-throughput? In particular, how can they be constructed to have precisely defined parameters including size, biomolecular composition and spatial organization? Microfluidic generation strategies have proved instrumental in addressing these questions. This article will outline some of the major principles underpinning membrane-based artificial cells and their construction using microfluidics, and will detail some recent landmarks that have been achieved.</jats:p

Crossref

Biochemical Society Transactions

Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology

http://www.biochemsoctrans.org/content/ppbiost/44/3/723.full.pdf

Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology

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