Catherine Gérard-Hirne

Eliane Keryer

Emmanuelle Issakidis-Bourguet

Isabelle Schepens

Jean-Pierre Jacquot

Mariana Stein

Myroslawa Miginiac-Maslow

Stéphane Lemaire

Abstract
               Heavy metals are highly toxic compounds for cells. In this report we demonstrate that the expression of Chlamydomonas reinhardtii thioredoxins (TRX)m and h is induced by heavy metals. Upon exposure of the cells to Cd and Hg, a strong accumulation of both messengers was observed. Western-blot experiments revealed that among these two TRXs, only TRX h polypeptides accumulated in response to the toxic cations. A biochemical analysis indicated that heavy metals inhibit TRX activity, presumably by binding at the level of their active site. Sequence analysis of the C. reinhardtii TRX h promoter revealed the presence of cis-acting elements related to cadmium induction. The origins and purposes of this regulation are discussed. Our data suggest, for the first time to our knowledge, a possible implication of TRXs in defense mechanisms against heavy metals.</jats:p

Stéphane Lemaire

Catherine Gérard-Hirne

Crossref

PLANT PHYSIOLOGY

Heavy-Metal Regulation of Thioredoxin Gene Expression in<i>Chlamydomonas reinhardtii</i>

http://www.plantphysiol.org/content/plantphysiol/120/3/773.full.pdf

Heavy-Metal Regulation of Thioredoxin Gene Expression in
                    Chlamydomonas reinhardtii

Heavy-Metal Regulation of Thioredoxin Gene Expression in Chlamydomonas reinhardtii

Abstract

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