A new Microcoryphian species is described from southern Spain. The new species belongs to the genus Promesomachilis and is named P. intermedia n. sp. because of the presence of intermediate characteristics in comparison with the other two species of the genus. The main features of the new species are: male maxillary palp with a field of spiralized setae on the ventral side of articles II-V, some setae also on article VI; second article of the male labial palp without a process on its distal part, though a little protuberant; labial palp also lacks a field of specialized setae, which are present in the two other species; each female gonapophysis with only 14-16 annuli, the lowest number ever found in the genus. The main feature that allow us to distinguish the three species is the presence of a sensorial field on femur II and III. In P. intermedia it is on the outer part of the femur, whereas in the other two species it is different.Se describe una nueva especie de Microcoryphia procedente del sur de España a la que denominamos Promesomachilis intermedia n. sp., por presentar caracteres intermedios entre las dos únicas que se conocían del género. La nueva especie puede caracterizarse por las siguientes particularidades: la parte ventral de los artejos II a V del palpo maxilar posee un campo de sedas espiraladas que también se encuentran, aunque en menor número, en el artejo VI; la parte dorsal del segundo artejo del palpo labial del macho no presenta ni la protuberancia (apenas perceptible en la nueva especie), ni el campo de sedas especiales que se hallan en las otras dos; las gonapófisis de la hembra constan de 14-16 divisiones, el menor número entre las tres especies. La principal característica que permite separar la nueva especie de las otras dos es la presencia, en los dos sexos, de un campo sensorial en la cara externa del fémur del segundo y tercer par de patas. En las otras especies, este campo está situado en otros pares de patas

Bach de Roca, Carmen

Gaju-Ricart, Miguel

Molero-Baltanás, Rafael

Paolo Fanciulli, Pietro

Orsis

Revistes Catalanes amb Accés Obert

Orsis 22, 2007  45-56A description of a new species of the genus
Promesomachilis from Spain (Insecta:
Microcoryphia)
Carmen Bach de Roca
Unitat de Zoologia, Departament de Biologia Animal, de Biologia Vegetal i d’Ecologia,
Universitat Autònoma de Barcelona. E-08193 Bellaterra, Barcelona, Spain
e-mail: Carmen.Bach@uab.es
Pietro Paolo Fanciulli
Dipartamento di Biologia Evolutiva, Università di Siena, Italy
Miguel Gaju-Ricart
Rafael Molero-Baltanás
Departamento de Zoología, Universidad de Córdoba, Spain
Manuscript received in September 2007
Abstract
A new Microcoryphian species is described from southern Spain. The new species belongs
to the genus Promesomachilis and is named P. intermedia n. sp. because of the presence
of intermediate characteristics in comparison with the other two species of the genus. The
main features of the new species are: male maxillary palp with a field of spiralized setae
on the ventral side of articles II-V, some setae also on article VI; second article of the male
labial palp without a process on its distal part, though a little protuberant; labial palp also
lacks a field of specialized setae, which are present in the two other species; each female
gonapophysis with only 14-16 annuli, the lowest number ever found in the genus. The main
feature that allow us to distinguish the three species is the presence of a sensorial field on
femur II and III. In P. intermedia it is on the outer part of the femur, whereas in the other
two species it is different.
Key words: Insecta, Microcoryphia, new species, Promesomachilis, Spain
Resumen. Descripción de una nueva especie de Promesomachilis de España (Insecta: Mi-
crocoryphia)
Se describe una nueva especie de Microcoryphia procedente del sur de España a la que
denominamos Promesomachilis intermedia n. sp., por presentar caracteres intermedios en-
tre las dos únicas que se conocían del género. La nueva especie puede caracterizarse por
las siguientes particularidades: la parte ventral de los artejos II a V del palpo maxilar po-
see un campo de sedas espiraladas que también se encuentran, aunque en menor número,
en el artejo VI; la parte dorsal del segundo artejo del palpo labial del macho no presenta
ni la protuberancia (apenas perceptible en la nueva especie), ni el campo de sedas espe-
46 Orsis 22, 2007 C. Bach de Roca; P. Paolo Fanciulli; M. Gaju-Ricart; R. Molero-Baltanásciales que se hallan en las otras dos; las gonapófisis de la hembra constan de 14-16 divi-
siones, el menor número entre las tres especies. La principal característica que permite se-
parar la nueva especie de las otras dos es la presencia, en los dos sexos, de un campo sensorial
en la cara externa del fémur del segundo y tercer par de patas. En las otras especies, este
campo está situado en otros pares de patas.
Palabras clave: Insecta, Microcoryphia, nueva especie, Promesomachilis, España.
Introduction
The genus Promesomachilis Silvestri, 1923 is endemic from the Iberian Penin-
sula and northwestern Morocco. Two species are known from the Iberian Penin-
sula: P. hispanica Silvestri, 1923 and P. cazorlensis Bach, 1983.
An allozyme electrophoresis analysis comparing three populations from south-
ern Spain (Fanciulli et al., 1995), revealed the presence of remarkable genetic dif-
ferentiation of the population collected in Alhaurín de la Torre (Málaga).
Morphological investigations on other specimens from Alhaurín de la Torre also
revealed some important differences that induced us to describe a new species:
P. intermedia n. sp.
Material and methods
The specimens were dissected and mounted in slides following the standard pro-
cedure. A recent description of this method could be seen in Molero et al. (2004).
Micrographs were obtained by means of scanning electron microscopy. Ma-
terial preserved in ethanol 80% was dehydrated in absolute ethanol, critical-point-
dried in a Balzer CPD 030 apparatus, and coated with gold in a Balzers MED 010
sputter coater. Observations were performed with a Philips XL20 scanning elec-
tron microscope.
Systematics
We describe a new species of Microcoryphia: Promesomachilis intermedia n. sp.
Description of the male
Body length: 9 mm; Length of the antennae (broken): 4 mm; length of paracer-
cus: 9mm; length of cerci: 4 mm.
Body and appendages are slightly pigmented. The head has pigmentation only
around the median ocellus: the front, that protudes between the ocelli, has a tiny
pigmentation. Ratio contact line/length of the eyes: 0.55, length/width of the eyes:
0.90 (Fig. 1A).
The antennae are longer than the body (as we have observed in another spec-
imen), scapus ratio maximum length/maximum width: 1.5-1.6; the distal chain
A new species of Promesomachilis from Spain Orsis 22, 2007  47Figure 1. Promesomachilis intermedia n. sp. male. A: Head in lateral view. B: Outline of
the labial palp. C: Sensory cones of the apex of third article of the labial palp. D: Maxillary
palp in external view. E: Setae of the ventral field of the maxillary palp. Scales: 0.1 mm.
A
D E
B C
48 Orsis 22, 2007 C. Bach de Roca; P. Paolo Fanciulli; M. Gaju-Ricart; R. Molero-Baltanáswith 14 annuli, each one show two rows of bristles with sensilla showing irreg-
ular distribution. The flagellum has alternatively white and brown segments (Figs.
2 A,B).
The maxillary palp shows very little brown pigment; it has normal setae and
a field of spiralized setae on the ventral side of articles II-V and some others on
article VI. The distribution of the hyaline striated spines on the last three articles
is: 5:3; 6:15; 7:12 (Figs. 1D, E, 2E). Ratio n/(n-1): 0.69.
The apical part of the third article of the labial palp is widened (typical of the
genus) and covered with normal bristles, sensory cones and small sensorial setae
(Figs. 1B, C, 2C, D). The second article, a little protruded in the distal part, lacks
special bristles or setae, as it occurs in the other two species.
The three pairs of legs show tiny macrochaetae on the coxa. The femur of the
foreleg is broader than in the other legs. Midleg without coxal stylet; the femur
has a row of sensilla in the outer ventral side. Hindleg with coxal stylet; femur
with a field of sensilla on the outer ventral side. The femur also has ciliary bris-
tles on his ventral side. In addition of the normal setae, all legs show spiniform
setae on the trochanter, femur, tibia and tarsomeres that, sometimes, appear as
spines. (Figs. 2F, 3A-C).
Urosternites II-V with acutangle sternite (Fig. 4A); VI-VII with rectangular
one; coxites II-V with two pairs of eversible vesicles; urosternite VIII without para-
meres; urosternite IX bear the penis and parameres (Fig. 3D). Ratio coxite/stylet
(without spine): II-VII: 1.5 -1.86; VIII: 1.21; IX:1.17; ratio spine/stylet (without
spine): II-VII: 0.30 – 0.38; VIII: 1.21; IX: 0.19.
Paramera IX with 1+6-7 divisions covered with small spines on his inner side
(Figs. 3D, 4B). Penis a little longer than the paramera, showing a circular open-
ing with bristles around the circle a papillae inside (Figs. 3D, 4C). Ratio basal
part/distal part: 1.12.
Cerci ending with two spines having different lengths (Fig. 3E).
Description of the female:
Body length: 10 mm; length of the antennae (broken): 4.5 mm; length of parac-
ercus: 9 mm; length of cerci: 3.5 mm.
The head shows similar features as the male. Eyes ratio contact line/length of
eyes: 0.52; length/width: 1.0.
Length and sensilla of the antennae as in the male.
The maxillary palp shows diffuse and uniform pigment. It lacks the field of
spiralized setae. The outline is as on Fig. 5 A. The distribution of the hyaline spines
on the last three articles is: 5:3; 6:16-20; 7:15-16. Ratio n/(n-1): 0.72.
The last article of the labial palp is slightly widened. Shape of sensory cones
as in the male (Fig. 5B).
All legs with macrochaeta on the dorsal side of the femur. Forelegs with a
slightly widened femur. Midleg and hindleg have a sensory field as in the male.
The three pair of legs bear spiniform setae on the ventral side of the femur, tib-
iae and tarsi (Figs. 5C-E, 6A-C).
A new species of Promesomachilis from Spain Orsis 22, 2007  49A
B
C
D
E
F
Figure 2. Promesomachilis intermedia n. sp male. A: Annuli of the antennal chain. Scale:
100 μm. B: Row of setae and sensilla of the annuli. Scale: 50 μm. C: Third article of the
labial palp. Scale: 200 μm. D: Sensory cones of the third article of the labial palp. Scale:
20 μm. E: Shape of the spines on the dorsal side of the 5th, 6th and 7th articles of the max-
illary palp. Scale: 60 μm. F: Femur of the 2nd pair of legs showing the field of sensory sen-
silla. Scale: 200 μm.
50 Orsis 22, 2007 C. Bach de Roca; P. Paolo Fanciulli; M. Gaju-Ricart; R. Molero-BaltanásFigure 3. Promesomachilis intermedia n. sp. male. A. First pair of legs. B: Second pair of
legs showing the sensorial field of sensilla on the femur. C: Id. third pair of legs. D: IX
urosternite with penis and paramera. E: End of the cerci. Scales: 0.1 mm.
A
B
C
D E
A new species of Promesomachilis from Spain Orsis 22, 2007  51B
A
CFigure 4. Promesomachilis intermedia n. sp male. A: Coxite of the V urosternite. Scale:
100 μm. B: Spines on the ventral side of paramera. Scale: 50 μm. C: Papilla inside the pe-
nis opening. Scale: 20 μm.
52 Orsis 22, 2007 C. Bach de Roca; P. Paolo Fanciulli; M. Gaju-Ricart; R. Molero-BaltanásFigure 5. Promesomachilis intermedia n. sp. female. A: Outline of the maxillary palp. B:
Id. labial palp. C: Id. first pair of legs. D: Id. second pair of legs showing the sensorial
field of sensilla. E: Id. Third pair of legs. F: VII urosternite. Scales: 0.1 mm.
A
B
C
D
E
F
A new species of Promesomachilis from Spain Orsis 22, 2007  53Urosternites as in the male. Sternites showing rectangular shape. Coxite VII
is ventrally prolonged between the stylets (Fig. 5F). Ratio length of the stylet (with-
out spine)/length of coxite: V-VII: 0.45-0.52; VIII: 0.71; IX: 0.61. Ratio length
of spine/length of stylet (without spine): II-VII: 0.33; VIII: 0.29; IX: 0.23.
The ovipositor is of the primary type (Sturm and Bach de Roca, 1992) and is
a little longer than the apex of the IX coxites. Gonapophysis VIII with 1+14-16
divisions all of them with long ciliary bristles as long as or longer than the width
of the division. Last division with 4-5 conules, the other divisions, from the apex,
have respectively 1-2, 1 and 1 conules (Figs. 6D-E, 7A).
Gonapophysis IX with 1+14-16 divisions all of them covered with ciliary bris-
tles as long or a little longer than the width of the division. The gonapophysis end
with a strong tooth. There are some spines on the ventral side (Figs. 6F, 7B). The
number of conules from the apex are: 4-5, 1-2, 1, 1, and 1.
End of the cerci as in the male.
Derivation of the name:
The new species is named Promesomachilis intermedia, because it shows inter-
mediate features in comparison to the other two species of the genus.
Type material:
Alhaurin de la Torre (Málaga) 1 male holotype + 6 males + 6 females paratypes.
Reference: M 507 (2 males + 2 females do not exist because have been used in
scanning electron microscopy), 29-03-93, Road C-344, Km 79 Arroyo Hondo; Al-
haurin de la Torre (Ma), 2 males paratypes, 14-02-93. Reference: M 888
Discussion
The genus Promesomachilis gives its name to a group of Microcoryphia genera
also including Bachilis, Catamachilis and Pseudocatamachilis (Sturm & Bach de
Roca, 1993). All of them are endemic in the Iberian Peninsula except Promeso-
machilis that was also found in the North Africa.
The presence of a sensorial field on the legs, observed for the first time in the
new species, led us to review also P. hispanica and the types of P. cazorlensis,
the other two species of the genus. This sensory field is also present in these species,
both in the males and in the females but it appears located in different legs in each
species (though it always exist on the P. II). On the basis of this character we can
distinguish the three species one from another (Table 1). In addition to this im-
portant character, the males also show other important morphological differences
on the maxillary and labial palps (see Table 1).
The following key can be used to distinguish the three species:
1.- Sensorial field present only on the external-ventral side of the femur of the
second pair of legs .............................. Promesomachilis hispanica Silvestri, 1923
54 Orsis 22, 2007 C. Bach de Roca; P. Paolo Fanciulli; M. Gaju-Ricart; R. Molero-BaltanásA
B
C F
D
E
Figure 6. Promesomachilis intermedia n. sp. female. A: Femur of the third pair of legs
showing the field of sensory sensilla. Scale: 200 μm. B: Sensory sensilla of the femur III.
Scale: 50 μm. C: One sensilla of the femur III. Scale: 5 μm. D: End of the VIII gonapophysis.
Scale: 100 μm. E: Special conules of the last annuli of the VIII gonapophysis, Scale: 5
μm. F: End of the IX gonapophysis. Scale: 50 μm.
A new species of Promesomachilis from Spain Orsis 22, 2007  551’.- Sensorial field present on femur of leg II and on another leg ...................... 2
2.- Sensorial field on femur leg I .......... Promesomachilis cazorlensis Bach, 1982
2’.- Sensorial field on femur leg III ................ Promesomachilis intermedia n. sp.
Aknowledgements
This work has been supported by the Project “Fauna Ibérica VII” (REN2001-1956-
C17-05).
References
Fanciulli, P. P.; Bach, C.; Gaju, M.; Frati, F. 1995. Genetic differentiation within two gen-
era of Microcoryphia (Insecta Apterygota): Promesomachilis and Praemachiloides. Bull.
Entomol. Pologne. 64: 33-40.
Molero-Baltanás, R.; Gaju-Ricart, M.; Bach de Roca, C. 2004. Hexápodos no insectos, mi-
crocorifios y zigentomados. In: Barrientos, J. A. (ed.) Curso práctico de Entomología.
Asociación española de Entomología, CIBIO, Manuals de la Universitat Autònoma de
Barcelona, 41: 471-496.
Sturm, H.; Bach de Roca, C. 1992. New American Meinertellidae (Archaeognatha,
Machiloidea). Pan-Pacific Entomol. 68: 174-191.
Sturm, H.; Bach de Roca, C. 1993. On the systematics of the Archaeognatha (Insecta). En-
tomol. Gener. 18: 55-90.Figure 7. Promesomachilis intermedia n. sp. female. A: VIII urosternite with gonapoph-
ysis. B: IX urosternite with gonapophysis. Scale: 0.1 mm.
A B
56 Orsis 22, 2007 C. Bach de Roca; P. Paolo Fanciulli; M. Gaju-Ricart; R. Molero-BaltanásTable 1. Main differences between the three species of Promesomachilis.
Character P. hispanica P. cazorlensis P. intermedia n.sp.
Number of annuli of distal
antennae chain
15-20 14 14
Ratio n/n-1 in maxillary palp 0.80 0.52 0.69-0.72
Field of setae in the ventral
side of maxillary palp
Small setae on
II-VI
Long ciliar
bristles on II-VI
Spiralized setae on
II-VI
Shape of 2nd article of labial
palp of male
With a a process
and a field of
setae on it
Without process
and with a field
of setae
Without process but
a little protruded
and without a field
of setae.
Femur leg I in males and
females
Without a
sensorial field
With a sensorial
field
Without a sensorial
field 
Femur leg III in males and
females
Without a
sensorial field
Without a
sensorial field
With a sensorial
field 
Number of annuli in
paramera IX
1+5-7 1+7-8 1+6-7
Ratio basal part of the penis/
distal part
1.12 1.18 1.12
Ovipositor length Longer than
coxite IX 
Shorter than
coxite IX 
Longer than coxite
IX 
Number of divisions of VIII
gonapophysis
19-20 21-23 14-16
Number of divisions of IX
gonapophysis
19-20 21-23 14-16
Geographical distribution South of Iberian
Peninsula and
North Africa
Cazorla
Mountains
(Jaen)
Malaga province


A description of a new species of the genus Promesomachilis from Spain (Insecta : Microcoryphia)

Diposit Digital de Documents de la UAB

Orsis 22, 2007  45-56A description of a new species of the genusPromesomachilis from Spain (Insecta:Microcoryphia)Carmen Bach de RocaUnitat de Zoologia, Departament de Biologia Animal, de Biologia Vegetal i d’Ecologia,Universitat Autònoma de Barcelona. E-08193 Bellaterra, Barcelona, Spaine-mail: Carmen.Bach@uab.esPietro Paolo FanciulliDipartamento di Biologia Evolutiva, Università di Siena, ItalyMiguel Gaju-RicartRafael Molero-BaltanásDepartamento de Zoología, Universidad de Córdoba, SpainManuscript received in September 2007AbstractA new Microcoryphian species is described from southern Spain. The new species belongsto the genus Promesomachilis and is named P. intermedia n. sp. because of the presenceof intermediate characteristics in comparison with the other two species of the genus. Themain features of the new species are: male maxillary palp with a field of spiralized setaeon the ventral side of articles II-V, some setae also on article VI; second article of the malelabial palp without a process on its distal part, though a little protuberant; labial palp alsolacks a field of specialized setae, which are present in the two other species; each femalegonapophysis with only 14-16 annuli, the lowest number ever found in the genus. The mainfeature that allow us to distinguish the three species is the presence of a sensorial field onfemur II and III. In P. intermedia it is on the outer part of the femur, whereas in the othertwo species it is different.Key words: Insecta, Microcoryphia, new species, Promesomachilis, SpainResumen. Descripción de una nueva especie de Promesomachilis de España (Insecta: Mi-crocoryphia)Se describe una nueva especie de Microcoryphia procedente del sur de España a la quedenominamos Promesomachilis intermedia n. sp., por presentar caracteres intermedios en-tre las dos únicas que se conocían del género. La nueva especie puede caracterizarse porlas siguientes particularidades: la parte ventral de los artejos II a V del palpo maxilar po-see un campo de sedas espiraladas que también se encuentran, aunque en menor número,en el artejo VI; la parte dorsal del segundo artejo del palpo labial del macho no presentani la protuberancia (apenas perceptible en la nueva especie), ni el campo de sedas espe-46 Orsis 22, 2007 C. Bach de Roca; P. Paolo Fanciulli; M. Gaju-Ricart; R. Molero-Baltanásciales que se hallan en las otras dos; las gonapófisis de la hembra constan de 14-16 divi-siones, el menor número entre las tres especies. La principal característica que permite se-parar la nueva especie de las otras dos es la presencia, en los dos sexos, de un campo sensorialen la cara externa del fémur del segundo y tercer par de patas. En las otras especies, estecampo está situado en otros pares de patas.Palabras clave: Insecta, Microcoryphia, nueva especie, Promesomachilis, España.IntroductionThe genus Promesomachilis Silvestri, 1923 is endemic from the Iberian Penin-sula and northwestern Morocco. Two species are known from the Iberian Penin-sula: P. hispanica Silvestri, 1923 and P. cazorlensis Bach, 1983.An allozyme electrophoresis analysis comparing three populations from south-ern Spain (Fanciulli et al., 1995), revealed the presence of remarkable genetic dif-ferentiation of the population collected in Alhaurín de la Torre (Málaga).Morphological investigations on other specimens from Alhaurín de la Torre alsorevealed some important differences that induced us to describe a new species:P. intermedia n. sp.Material and methodsThe specimens were dissected and mounted in slides following the standard pro-cedure. A recent description of this method could be seen in Molero et al. (2004).Micrographs were obtained by means of scanning electron microscopy. Ma-terial preserved in ethanol 80% was dehydrated in absolute ethanol, critical-point-dried in a Balzer CPD 030 apparatus, and coated with gold in a Balzers MED 010sputter coater. Observations were performed with a Philips XL20 scanning elec-tron microscope.SystematicsWe describe a new species of Microcoryphia: Promesomachilis intermedia n. sp.Description of the maleBody length: 9 mm; Length of the antennae (broken): 4 mm; length of paracer-cus: 9mm; length of cerci: 4 mm.Body and appendages are slightly pigmented. The head has pigmentation onlyaround the median ocellus: the front, that protudes between the ocelli, has a tinypigmentation. Ratio contact line/length of the eyes: 0.55, length/width of the eyes:0.90 (Fig. 1A).The antennae are longer than the body (as we have observed in another spec-imen), scapus ratio maximum length/maximum width: 1.5-1.6; the distal chainA new species of Promesomachilis from Spain Orsis 22, 2007  47Figure 1. Promesomachilis intermedia n. sp. male. A: Head in lateral view. B: Outline ofthe labial palp. C: Sensory cones of the apex of third article of the labial palp. D: Maxillarypalp in external view. E: Setae of the ventral field of the maxillary palp. Scales: 0.1 mm.AD EB C48 Orsis 22, 2007 C. Bach de Roca; P. Paolo Fanciulli; M. Gaju-Ricart; R. Molero-Baltanáswith 14 annuli, each one show two rows of bristles with sensilla showing irreg-ular distribution. The flagellum has alternatively white and brown segments (Figs.2 A,B).The maxillary palp shows very little brown pigment; it has normal setae anda field of spiralized setae on the ventral side of articles II-V and some others onarticle VI. The distribution of the hyaline striated spines on the last three articlesis: 5:3; 6:15; 7:12 (Figs. 1D, E, 2E). Ratio n/(n-1): 0.69.The apical part of the third article of the labial palp is widened (typical of thegenus) and covered with normal bristles, sensory cones and small sensorial setae(Figs. 1B, C, 2C, D). The second article, a little protruded in the distal part, lacksspecial bristles or setae, as it occurs in the other two species.The three pairs of legs show tiny macrochaetae on the coxa. The femur of theforeleg is broader than in the other legs. Midleg without coxal stylet; the femurhas a row of sensilla in the outer ventral side. Hindleg with coxal stylet; femurwith a field of sensilla on the outer ventral side. The femur also has ciliary bris-tles on his ventral side. In addition of the normal setae, all legs show spiniformsetae on the trochanter, femur, tibia and tarsomeres that, sometimes, appear asspines. (Figs. 2F, 3A-C).Urosternites II-V with acutangle sternite (Fig. 4A); VI-VII with rectangularone; coxites II-V with two pairs of eversible vesicles; urosternite VIII without para-meres; urosternite IX bear the penis and parameres (Fig. 3D). Ratio coxite/stylet(without spine): II-VII: 1.5 -1.86; VIII: 1.21; IX:1.17; ratio spine/stylet (withoutspine): II-VII: 0.30 – 0.38; VIII: 1.21; IX: 0.19.Paramera IX with 1+6-7 divisions covered with small spines on his inner side(Figs. 3D, 4B). Penis a little longer than the paramera, showing a circular open-ing with bristles around the circle a papillae inside (Figs. 3D, 4C). Ratio basalpart/distal part: 1.12.Cerci ending with two spines having different lengths (Fig. 3E).Description of the female:Body length: 10 mm; length of the antennae (broken): 4.5 mm; length of parac-ercus: 9 mm; length of cerci: 3.5 mm.The head shows similar features as the male. Eyes ratio contact line/length ofeyes: 0.52; length/width: 1.0.Length and sensilla of the antennae as in the male.The maxillary palp shows diffuse and uniform pigment. It lacks the field ofspiralized setae. The outline is as on Fig. 5 A. The distribution of the hyaline spineson the last three articles is: 5:3; 6:16-20; 7:15-16. Ratio n/(n-1): 0.72.The last article of the labial palp is slightly widened. Shape of sensory conesas in the male (Fig. 5B).All legs with macrochaeta on the dorsal side of the femur. Forelegs with aslightly widened femur. Midleg and hindleg have a sensory field as in the male.The three pair of legs bear spiniform setae on the ventral side of the femur, tib-iae and tarsi (Figs. 5C-E, 6A-C).A new species of Promesomachilis from Spain Orsis 22, 2007  49ABCDEFFigure 2. Promesomachilis intermedia n. sp male. A: Annuli of the antennal chain. Scale:100 μm. B: Row of setae and sensilla of the annuli. Scale: 50 μm. C: Third article of thelabial palp. Scale: 200 μm. D: Sensory cones of the third article of the labial palp. Scale:20 μm. E: Shape of the spines on the dorsal side of the 5th, 6th and 7th articles of the max-illary palp. Scale: 60 μm. F: Femur of the 2nd pair of legs showing the field of sensory sen-silla. Scale: 200 μm.50 Orsis 22, 2007 C. Bach de Roca; P. Paolo Fanciulli; M. Gaju-Ricart; R. Molero-BaltanásFigure 3. Promesomachilis intermedia n. sp. male. A. First pair of legs. B: Second pair oflegs showing the sensorial field of sensilla on the femur. C: Id. third pair of legs. D: IXurosternite with penis and paramera. E: End of the cerci. Scales: 0.1 mm.ABCD EA new species of Promesomachilis from Spain Orsis 22, 2007  51BACFigure 4. Promesomachilis intermedia n. sp male. A: Coxite of the V urosternite. Scale:100 μm. B: Spines on the ventral side of paramera. Scale: 50 μm. C: Papilla inside the pe-nis opening. Scale: 20 μm.52 Orsis 22, 2007 C. Bach de Roca; P. Paolo Fanciulli; M. Gaju-Ricart; R. Molero-BaltanásFigure 5. Promesomachilis intermedia n. sp. female. A: Outline of the maxillary palp. B:Id. labial palp. C: Id. first pair of legs. D: Id. second pair of legs showing the sensorialfield of sensilla. E: Id. Third pair of legs. F: VII urosternite. Scales: 0.1 mm.ABCDEFA new species of Promesomachilis from Spain Orsis 22, 2007  53Urosternites as in the male. Sternites showing rectangular shape. Coxite VIIis ventrally prolonged between the stylets (Fig. 5F). Ratio length of the stylet (with-out spine)/length of coxite: V-VII: 0.45-0.52; VIII: 0.71; IX: 0.61. Ratio lengthof spine/length of stylet (without spine): II-VII: 0.33; VIII: 0.29; IX: 0.23.The ovipositor is of the primary type (Sturm and Bach de Roca, 1992) and isa little longer than the apex of the IX coxites. Gonapophysis VIII with 1+14-16divisions all of them with long ciliary bristles as long as or longer than the widthof the division. Last division with 4-5 conules, the other divisions, from the apex,have respectively 1-2, 1 and 1 conules (Figs. 6D-E, 7A).Gonapophysis IX with 1+14-16 divisions all of them covered with ciliary bris-tles as long or a little longer than the width of the division. The gonapophysis endwith a strong tooth. There are some spines on the ventral side (Figs. 6F, 7B). Thenumber of conules from the apex are: 4-5, 1-2, 1, 1, and 1.End of the cerci as in the male.Derivation of the name:The new species is named Promesomachilis intermedia, because it shows inter-mediate features in comparison to the other two species of the genus.Type material:Alhaurin de la Torre (Málaga) 1 male holotype + 6 males + 6 females paratypes.Reference: M 507 (2 males + 2 females do not exist because have been used inscanning electron microscopy), 29-03-93, Road C-344, Km 79 Arroyo Hondo; Al-haurin de la Torre (Ma), 2 males paratypes, 14-02-93. Reference: M 888DiscussionThe genus Promesomachilis gives its name to a group of Microcoryphia generaalso including Bachilis, Catamachilis and Pseudocatamachilis (Sturm & Bach deRoca, 1993). All of them are endemic in the Iberian Peninsula except Promeso-machilis that was also found in the North Africa.The presence of a sensorial field on the legs, observed for the first time in thenew species, led us to review also P. hispanica and the types of P. cazorlensis,the other two species of the genus. This sensory field is also present in these species,both in the males and in the females but it appears located in different legs in eachspecies (though it always exist on the P. II). On the basis of this character we candistinguish the three species one from another (Table 1). In addition to this im-portant character, the males also show other important morphological differenceson the maxillary and labial palps (see Table 1).The following key can be used to distinguish the three species:1.- Sensorial field present only on the external-ventral side of the femur of thesecond pair of legs .............................. Promesomachilis hispanica Silvestri, 192354 Orsis 22, 2007 C. Bach de Roca; P. Paolo Fanciulli; M. Gaju-Ricart; R. Molero-BaltanásABC FDEFigure 6. Promesomachilis intermedia n. sp. female. A: Femur of the third pair of legsshowing the field of sensory sensilla. Scale: 200 μm. B: Sensory sensilla of the femur III.Scale: 50 μm. C: One sensilla of the femur III. Scale: 5 μm. D: End of the VIII gonapophysis.Scale: 100 μm. E: Special conules of the last annuli of the VIII gonapophysis, Scale: 5μm. F: End of the IX gonapophysis. Scale: 50 μm.A new species of Promesomachilis from Spain Orsis 22, 2007  551’.- Sensorial field present on femur of leg II and on another leg ...................... 22.- Sensorial field on femur leg I .......... Promesomachilis cazorlensis Bach, 19822’.- Sensorial field on femur leg III ................ Promesomachilis intermedia n. sp.AknowledgementsThis work has been supported by the Project “Fauna Ibérica VII” (REN2001-1956-C17-05).ReferencesFanciulli, P. P.; Bach, C.; Gaju, M.; Frati, F. 1995. Genetic differentiation within two gen-era of Microcoryphia (Insecta Apterygota): Promesomachilis and Praemachiloides. Bull.Entomol. Pologne. 64: 33-40.Molero-Baltanás, R.; Gaju-Ricart, M.; Bach de Roca, C. 2004. Hexápodos no insectos, mi-crocorifios y zigentomados. In: Barrientos, J. A. (ed.) Curso práctico de Entomología.Asociación española de Entomología, CIBIO, Manuals de la Universitat Autònoma deBarcelona, 41: 471-496.Sturm, H.; Bach de Roca, C. 1992. New American Meinertellidae (Archaeognatha,Machiloidea). Pan-Pacific Entomol. 68: 174-191.Sturm, H.; Bach de Roca, C. 1993. On the systematics of the Archaeognatha (Insecta). En-tomol. Gener. 18: 55-90.Figure 7. Promesomachilis intermedia n. sp. female. A: VIII urosternite with gonapoph-ysis. B: IX urosternite with gonapophysis. Scale: 0.1 mm.A B56 Orsis 22, 2007 C. Bach de Roca; P. Paolo Fanciulli; M. Gaju-Ricart; R. Molero-BaltanásTable 1. Main differences between the three species of Promesomachilis.Character P. hispanica P. cazorlensis P. intermedia n.sp.Number of annuli of distalantennae chain15-20 14 14Ratio n/n-1 in maxillary palp 0.80 0.52 0.69-0.72Field of setae in the ventralside of maxillary palpSmall setae onII-VILong ciliarbristles on II-VISpiralized setae onII-VIShape of 2nd article of labialpalp of maleWith a a processand a field ofsetae on itWithout processand with a fieldof setaeWithout process buta little protrudedand without a fieldof setae.Femur leg I in males andfemalesWithout asensorial fieldWith a sensorialfieldWithout a sensorialfield Femur leg III in males andfemalesWithout asensorial fieldWithout asensorial fieldWith a sensorialfield Number of annuli inparamera IX1+5-7 1+7-8 1+6-7Ratio basal part of the penis/distal part1.12 1.18 1.12Ovipositor length Longer thancoxite IX Shorter thancoxite IX Longer than coxiteIX Number of divisions of VIIIgonapophysis19-20 21-23 14-16Number of divisions of IXgonapophysis19-20 21-23 14-16Geographical distribution South of IberianPeninsula andNorth AfricaCazorlaMountains(Jaen)Malaga province

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A description of a new species of the genus Promesomachilis from Spain (Insecta : Microcoryphia)

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