AbstractWe have developed procedures to solubilize the envelope UDP-galactose:diacylglycerol galactosyltransferase activity and to assay this enzyme after solubilization with a zwitterionic non-denaturing detergent (CHAPS) and fractionation. From solubilized envelope membranes isolated from intact spinach chloroplasts, we were able to prepare by chromatography on hydroxyapatite a fraction enriched (6–7-fold) in this enzyme responsible for monogalactosyldiacylglycerol synthesis
