Here we classify endocytic structures at the adherent (bottom) surface of many cells in culture into shorter-lived coated pits and longer-lived coated plaques which internalize by different mechanisms

Saveez Saffarian

Emanuele Cocucci

Tomas Kirchhausen

Fred Hughson

T. J Brett

S. D Conner

T Kirchhausen

M Kaksonen

M Ehrlich

I Gaidarov

P. A Keyel

C Le Clainche

C. J Merrifield

T. M Newpher

J. Z Rappoport

S Saffarian

D Yarar

R Zoncu

D Perrais

Y Cheng

A Fotin

R. H Massol

P Maupin

J Aggeler

T Akisaka

J Heuser

K. D Bellve

A. E. Y Engqvist-Goldstein

M. I Geli

L. M Fujimoto

E Boucrot

S Benesch

D. K Cureton

A Shimada

E Macia

J. H Hartwig

A Motley

E Ungewickell

D-w Lee

C-Y Chen

V Legendre-Guillemin

V Poupon

A. M Weaver

H Higgs

T Uruno

M. A McNiven

F Huang

H Chen

M. A Edeling

F Tebar

H Wang

M. J Hawryluk

E Veiga

M. G. J Ford

C. P Toret

F-Z Idrissi

M Sachse

English

PubMed

Saffarian Saveez

Cocucci Emanuele

Kirchhausen Tomas

Public Library of Science (PLOS)

Distinct Dynamics of Endocytic Clathrin-Coated Pits and Coated Plaques

Crossref

Clathrin is the scaffold of a conserved molecular machinery that has evolved to capture membrane patches, which then pinch off to become traffic carriers. These carriers are the principal vehicles of receptor-mediated endocytosis and are the major route of traffic from plasma membrane to endosomes. We report here the use of in vivo imaging data, obtained from spinning disk confocal and total internal reflection fluorescence microscopy, to distinguish between two modes of endocytic clathrin coat formation, which we designate as "coated pits" and "coated plaques." Coated pits are small, rapidly forming structures that deform the underlying membrane by progressive recruitment of clathrin, adaptors, and other regulatory proteins. They ultimately close off and bud inward to form coated vesicles. Coated plaques are longer-lived structures with larger and less sharply curved coats; their clathrin lattices do not close off, but instead move inward from the cell surface shortly before membrane fission. Local remodeling of actin filaments is essential for the formation, inward movement, and dissolution of plaques, but it is not required for normal formation and budding of coated pits in the cells we have studied. We conclude that there are at least two distinct modes of clathrin coat formation at the plasma membrane--classical coated pits and coated plaques--and that these two assemblies interact quite differently with other intracellular structures

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PLoS Biology

Distinct dynamics of endocytic clathrin-coated pits and coated plaques.

Desde comienzos del nuevo milenio se viene construyendo en América del Sur, un nuevo proceso de integración regional. Por sus particularidades, se trata de un proceso que desbor- da los modelos explicativos existentes. De allí la aparición de distintas propuestas de carac- terización, como la del regionalismo post-neoliberal o la del post-hegemónico. Sin embargo, ninguna de ellas ha logrado aún una síntesis acabada del fenómeno sudamericano

Nicolás Falomir Lockhart

Red de Bibliotecas Virtuales de Ciencias Sociales de América Latina y El Caribe

LA IDENTIDAD DE UNASUR: ¿REGIONALISMO POST-NEOLIBERAL O POST-HEGEMÓNICO?

Since the new millennium, a new process of regional integration is being built in South America. However, due to its particularities it is a process that cannot be explained by the pre-existing models of integration. Therefore, many conceptualizations have been proposed, such as post-neoliberal regionalism or post-hegemonic regionalism. Yet, none of them has reached a successful synthesis of the South American phenomenon.Desde comienzos del nuevo milenio se viene construyendo en América del Sur, un nuevo proceso de integración regional. Por sus particularidades, se trata de un proceso que desborda los modelos explicativos existentes. De allí la aparición de distintas propuestas de caracterización, como la del regionalismo post-neoliberal o la del post-hegemónico. Sin embargo, ninguna de ellas ha logrado aún una síntesis acabada del fenómeno sudamericano

Falomir Lockhart, Nicolás

Kerwa repositorio (Universidad de Costa Rica)

Name not available

A burst of auxilin recruitment determines the onset of clathrin-coated vesicle uncoating.

A dynamic actin cytoskeleton functions at multiple stages of clathrin-mediated endocytosis.

A Hip1R-cortactin complex negatively regulates actin assembly associated with endocytosis.

A modular design for the clathrinand actin-mediated endocytosis machinery.

Actin assembly and endocytosis: from yeast to mammals.

Actin assembly plays a variable, but not obligatory role in receptor-mediated endocytosis.

Activation of Arp2/3 complex-mediated actin polymerization by cortactin.

Adhesion structures and their cytoskeleton-membrane interactions at podosomes of osteoclasts in culture.

An ultrastructural approach to understanding the cytoskeleton. In: Carraway KL, Carraway CAC,

Analysis of the AP-2 adaptor complex and cargo during clathrin-mediated endocytosis.

Bilayered clathrin coats on endosomal vacuoles are involved in protein sorting toward lysosomes.

Branching out: cortactin stabilizes actin networks generated by the Arp2/3 complex.

Clathrin light chains function in mannose phosphate receptor trafficking via regulation of actin assembly.

Clathrin sheets on the protoplasmic surface of ventral membranes of osteoclasts in culture.

Clathrin-mediated endocytosis in AP-2-depleted cells.

Clathrinmediated endocytosis of the epithelial sodium channel: role of epsin.

Cortactin promotes and stabilizes Arp2/3-induced actin filament network formation.

Coupling between clathrin-coated-pit invagination, cortactin recruitment, and membrane scission observed in live cells.

Cryo-electron tomography of clathrin-coated vesicles: structural implications for coat assembly.

Curvature of clathrin-coated pits driven by epsin.

Curved EFC/ F-BAR-domain dimers are joined end to end into a filament for membrane invagination in endocytosis.

Differential evanescence nanometry: live-cell fluorescence measurements with 10-nm axial resolution on the plasma membrane.

Distinct acto/myosin-I structures associate with endocytic profiles at the plasma membrane.

Dynamics of clathrin and adaptor proteins during endocytosis.

Dynamics of endocytic vesicle creation.

Dynasore, a cell-permeable inhibitor of dynamin.

Effects of cytoplasmic acidification on clathrin lattice morphology.

Endocytic adaptor molecules reveal an endosomal population of clathrin by total internal reflection fluorescence microscopy.

Endocytosis by random initiation and stabilization of clathrin-coated pits.

Eps15 is a component of clathrin-coated pits and vesicles and is located at the rim of coated pits.

Epsin 1 is a polyubiquitin-selective clathrin-associated sorting protein.

Epsin is an EHdomain-binding protein implicated in clathrin-mediated endocytosis.

Harnessing actin dynamics for clathrin-mediated endocytosis.

Huntingtin interacting protein 1 (HIP1) regulates clathrin assembly through Clathrin Coat Dynamics during Endocytosis PLoS

Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo.

Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits.

Improved preservation and staining of HeLa cell actin filaments, clathrin-coated membranes, and other cytoplasmic structures by tannic acid-glutaraldehyde-saponin fixation.

In vivo dynamics of clathrin and its adaptor-dependent recruitment to the actin-based endocytic machinery in yeast.

Initial events during phagocytosis by macrophages viewed from outside and inside the cell: membrane-particle interactions and clathrin.

Invasive and adherent bacterial pathogens co-opt host clathrin for infection.

Listeria hijacks the clathrin-dependent endocytic machinery to invade mammalian cells.

Loss of endocytic clathrin-coated pits upon acute depletion of phosphatidylinositol 4,5-bisphosphate.

Molecular model for a complete clathrin lattice from electron cryomicroscopy.

Molecular structures of coat and coat-associated proteins: function follows form.

Molecular switches involving the AP-2 [beta]2 appendage regulate endocytic cargo selection and clathrin coat assembly.

Multiple pathways regulate endocytic coat disassembly in Saccharomyces cerevisiae for optimal downstream trafficking.

N-WASP deficiency impairs EGF internalization and actin assembly at clathrin-coated pits.

Neural Wiskott Aldrich Syndrome Protein (N-WASP) and the Arp2/3 complex are recruited to sites of clathrin-mediated endocytosis in cultured fibroblasts.

Plasma membrane domains specialized for clathrin-mediated endocytosis in primary cells.

Recruitment dynamics of GAK and auxilin to clathrin-coated pits during endocytosis.

Regulated interactions between dynamin and the actin-binding protein cortactin modulate cell shape.

Regulated portals of entry into the cell.

RNAi-mediated Hip1R silencing results in stable association between the endocytic machinery and the actin assembly machinery.

Role of auxilin in uncoating clathrin-coated vesicles.

Role of lipids and actin in the formation of clathrin-coated pits.

Role of type i myosins in receptor-mediated endocytosis in yeast.

Sorkin A

Spatial control of coated-pit dynamics in living cells.

Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating.

The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro.

The association of epsin with ubiquitinated cargo along the endocytic pathway is negatively regulated by its interaction with clathrin.

The role of clathrin-dependent endocytosis in bacterial internalization.

Three-dimensional visualization of coated vesicle formation in fibroblasts.

Vesicular stomatitis virus enters cells through vesicles incompletely coated with clathrin that depend upon actin for internalization. PLoS Pathog 5:

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2731173

Distinct Dynamics of Endocytic Clathrin-Coated Pits and Coated Plaques

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