Abstract. Abstract syntax with variable binding is known to be characterised as an initial algebra in a presheaf category. This paper extends it to the case of poly-morphic typed abstract syntax with binding. We consider two variations, second-order and higher-order polymorphic syntax. The central idea is to apply Fiore’s initial algebra characterisation of typed abstract syntax with binding repeatedly, i.e. first to the type structure and secondly to the term structure of polymorphic system. In this process, we use the Grothendieck construction to combine differ-ently staged categories of polymorphic contexts.