Bohus, B.,

Driscoll, P.,

Koolhaas, J.M.,

Roozendaal, B.,

Schoorlemmer, G.H.M.,

Sluyter, S.,

Wiersma, A.,

English

ROOZENDAAL, B

SCHOORLEMMER, GHM

WIERSMA, A

SLUYTER, S

DRISCOLL, P

KOOLHAAS, JM

BOHUS, B

University of Groningen Research Database

OPPOSITE EFFECTS OF CENTRAL AMYGDALOID VASOPRESSIN AND OXYTOCIN ON THE REGULATION OF CONDITIONED STRESS RESPONSES IN MALE-RATS

Roozendaal, B.

Schoorlemmer, G.H.M.

Wiersma, A.

Sluyter, S.

Driscoll, P.

Koolhaas, J.M.

Bohus, B.

NARCIS 

Opposite effects of central amygdaloid vasopressin and oxytocin on the regulation of conditioned stress responses in male rats

University of Groningen Digital Archive

Opposite Effects of Central 
Amygdaloid Vasopressin and 
Oxytocin on the Regulation of 
Conditioned Stress Responses in 
Male Rats 
3. ROOZENDAAL,asb  G. H.  M.  SCHOORLEMMERP 
A. WIERSMA,'  S.  SLUYTER," P. DRISCOLL,' 
J.  M.  KOOLHAASP AND 3.  BOHUS ' 
'Department  of  Animal Physiologv 
University of  Groningen 
Centre for Behavioural, Cognitive and Neuro-Sciences 
9750 AA Haren, the Netherlands 
'Behavioral Biologv Laboratory 
ETHZ 
CH-8092 Ziirich, Switzerland 
The central nucleus of  the amygdala (CEA) seems to be selectively involved in the 
passive component of  the behavioral (immobility) and the accompanying parasympa- 
thetic response (bradycardia) during conditioned environmental challenges,' leaving 
conditioned sympathetic, adreno-medullary, and adreno-cortical responses unaffected 
(Roozendaal et al., in preparation). Robust monosynaptic, .peptidergic connections to 
the vagal complex and periaqueductal gray may be mediating these effects. 
Intracerebroventricularly administered arginine-8-vasopressin (AVP) potentiates 
these stress-induced bradycardiac and immobility responses.* AVP may exert these 
effects via AVP and/or  oxytocin (OT) receptive systems in the CEA.' Microinfusion 
of AVP (dissolved in 1 p1  artificial CSF) into the CEA of conscious male Wistar rats 
under resting conditions, leads to a dose-dependent change in heart rate and behavior: 
A high dose of  AVP (2 ng), but not lower ones (20 and 200 pg),  causes a transient 
increase in heart rate and behavioral a~tivity.~  Moreover, in a part of the animals, the 
low dose of AVP even causes an opposite effect, that is, bradycardia. OT  administration 
(2 ng) induces responses similar to those induced with the high dose of  AVP. Neither 
plasma norepinephrine nor epinephrine is influenced after AVP infusion, suggesting 
that the tachycardia is due to reduced parasympathetic control, rather than a stimula- 
tion of the sympathetic outflow. The effects of AVP and OT during conditioned stress 
were studied in male Roman high-avoidance (RHA) and Roman low-avoidance. (RLA) 
rats, selected genetically for shuttle-box acquisition behavior. In RLA rats the cardiac 
and behavioral responses to the emotional stressor are bradycardia and immobility, 
'Address  for correspondence: Department of  Animal Physiology, University of Groningen, 
Centre for Behavioural, Cognitive  and Neuro-Sciences,  P.O. Box 14,9750  AA Haren, the Nether- 
lands. 
460 ROOZENDAAL et al.: REGULATION OF STRESS RESPONSE  461 
suggesting an important role of the CEA in these rats. The RHA rats, however, fail to 
show  any  change in  heart  rate or immobility in response to a  conditioned  stress 
situation. The low dose of AVP in the CEA of conscious RLA rats causes an enhance- 
ment of the stress-induced bradycardiac and immobility response. However, the high 
dose of  AVP (2 ng)  and  OT (200  pg)  attenuate the bradycardiac  and  immobility 
response in the RLA rats. As expected, infusion of AVP and OT in the RHA rats fails 
to induce any change in heart  rate or immobility. Binding studies revealed that in 
contrast to the selective and highly potent AVP receptor, the OT receptor recognizes 
AVP and OT with similar affinit~.~  This suggests that the behavioral and autonomic 
responses of  the high dose of  AVP may be caused by OT receptor activation. 
In conclusion, central  amygdaloid vasopressinergic and oxytocinergic receptor- 
mediated mechanisms seem to exert opposite effects on the regulation of stress-induced 
autonomic and behavioral responses. AVP enhances the passive components of  the 
conditioned stress response. The oxytocinergic system attenuates the CEA output. The 
stimulating role of AVP in passive coping may be general and has also been reported 
in other brain regions. The differences between RLA and RHA males suggest that 
these peptidergic mechanisms are only active in rats that preferentially adopt a passive 
coping strategy.2 
REFERENCES 
1.  ROOZENDAAL,  B., J. M. KOOLHAAS  & B.  BOHUS.  1990. Behav. Brain Res. 41:  39-48. 
2.  BOHUS,  B., J. M. KOOLHAAS,  P. G. M. LUITEN,  C. A. M. VERSTEEG,  S.  M. KORTE  & D. 
JAARSMA.  1989. Vasopressin and related peptides: Involvement in central cardiovascular 
regulation. In Hypertension, Brain Catecholamines and Peptides. F.  P. Nykamp & D. de 
Wied, Eds.: 99- 110. Elsevier. Amsterdam. 
3.  TRIBOLLET,  E., C. BARBERIS,  S. JARD,  M. DUBOIS-DAUPHIN  & J. J. DREIFUSS.  1988. Brain 
Res. 442: 105-118. 
4.  ROOZENDAAL,  B., G. H. M. SCHOORLEMMER,  J. M. KOOLHAAS  & B.  BOHUS.  1990. 20th 
Annual Meeting of the Society of  Neuroscience, St. Louis, MO (USA). Abstr. 221.14. 
5.  ELANDS,  J., C. BARBERIS  & S. JARD.  1988. Am. J. Physiol. 254 E31-E38. 

Vasopressin and related peptides: Involvement in central cardiovascular regulation.

Opposite Effects of Central Amygdaloid Vasopressin and Oxytocin on the Regulation of Conditioned Stress Responses in Male Rats

  
 University of Groningen
Opposite Effects of Central Amygdaloid Vasopressin and Oxytocin on the Regulation of
Conditioned Stress Responses in Male Rats
Roozendaal, B.; Schoorlemmer, G.H.M.; Wiersma, A.; Sluyter, S.; Driscoll, P.; Koolhaas,
J.M.; Bohus, B.
Published in:
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
DOI:
10.1111/j.1749-6632.1992.tb34384.x
IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from
it. Please check the document version below.
Document Version
Publisher's PDF, also known as Version of record
Publication date:
1992
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Roozendaal, B., Schoorlemmer, G. H. M., Wiersma, A., Sluyter, S., Driscoll, P., Koolhaas, J. M., & Bohus,
B. (1992). Opposite Effects of Central Amygdaloid Vasopressin and Oxytocin on the Regulation of
Conditioned Stress Responses in Male Rats. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES,
652(1), 460-461. DOI: 10.1111/j.1749-6632.1992.tb34384.x
Copyright
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Download date: 10-02-2018
Opposite Effects of Central 
Amygdaloid Vasopressin and 
Oxytocin on the Regulation of 
Conditioned Stress Responses in 
Male Rats 
3. ROOZENDAAL,asb G. H. M. SCHOORLEMMERP 
A. WIERSMA,' S. SLUYTER," P. DRISCOLL,' 
J. M. KOOLHAASP AND 3. BOHUS ' 
'Department of Animal Physiologv 
University of Groningen 
Centre for Behavioural, Cognitive and Neuro-Sciences 
9750 AA Haren, the Netherlands 
'Behavioral Biologv Laboratory 
ETHZ 
CH-8092 Ziirich, Switzerland 
The central nucleus of the amygdala (CEA) seems to be selectively involved in the 
passive component of the behavioral (immobility) and the accompanying parasympa- 
thetic response (bradycardia) during conditioned environmental challenges,' leaving 
conditioned sympathetic, adreno-medullary, and adreno-cortical responses unaffected 
(Roozendaal et al., in preparation). Robust monosynaptic, .peptidergic connections to 
the vagal complex and periaqueductal gray may be mediating these effects. 
Intracerebroventricularly administered arginine-8-vasopressin (AVP) potentiates 
these stress-induced bradycardiac and immobility responses.* AVP may exert these 
effects via AVP and/or oxytocin (OT) receptive systems in the CEA.' Microinfusion 
of AVP (dissolved in 1 p1 artificial CSF) into the CEA of conscious male Wistar rats 
under resting conditions, leads to a dose-dependent change in heart rate and behavior: 
A high dose of AVP (2 ng), but not lower ones (20 and 200 pg), causes a transient 
increase in heart rate and behavioral a~t iv i ty .~  Moreover, in a part of the animals, the 
low dose of AVP even causes an opposite effect, that is, bradycardia. OT administration 
(2 ng) induces responses similar to those induced with the high dose of AVP. Neither 
plasma norepinephrine nor epinephrine is influenced after AVP infusion, suggesting 
that the tachycardia is due to reduced parasympathetic control, rather than a stimula- 
tion of the sympathetic outflow. The effects of AVP and OT during conditioned stress 
were studied in male Roman high-avoidance (RHA) and Roman low-avoidance. (RLA) 
rats, selected genetically for shuttle-box acquisition behavior. In RLA rats the cardiac 
and behavioral responses to the emotional stressor are bradycardia and immobility, 
'Address for correspondence: Department of Animal Physiology, University of Groningen, 
Centre for Behavioural, Cognitive and Neuro-Sciences, P.O. Box 14,9750 AA Haren, the Nether- 
lands. 
460 
ROOZENDAAL et al.: REGULATION OF STRESS RESPONSE 461 
suggesting an important role of the CEA in these rats. The RHA rats, however, fail to 
show any change in heart rate or immobility in response to a conditioned stress 
situation. The low dose of AVP in the CEA of conscious RLA rats causes an enhance- 
ment of the stress-induced bradycardiac and immobility response. However, the high 
dose of AVP (2 ng) and OT (200 pg) attenuate the bradycardiac and immobility 
response in the RLA rats. As expected, infusion of AVP and OT in the RHA rats fails 
to induce any change in heart rate or immobility. Binding studies revealed that in 
contrast to the selective and highly potent AVP receptor, the OT receptor recognizes 
AVP and OT with similar affinit~.~ This suggests that the behavioral and autonomic 
responses of the high dose of AVP may be caused by OT receptor activation. 
In conclusion, central amygdaloid vasopressinergic and oxytocinergic receptor- 
mediated mechanisms seem to exert opposite effects on the regulation of stress-induced 
autonomic and behavioral responses. AVP enhances the passive components of the 
conditioned stress response. The oxytocinergic system attenuates the CEA output. The 
stimulating role of AVP in passive coping may be general and has also been reported 
in other brain regions. The differences between RLA and RHA males suggest that 
these peptidergic mechanisms are only active in rats that preferentially adopt a passive 
coping strategy.2 
REFERENCES 
1. ROOZENDAAL, B., J. M. KOOLHAAS & B. BOHUS. 1990. Behav. Brain Res. 41: 39-48. 
2. BOHUS, B., J. M. KOOLHAAS, P. G. M. LUITEN, C. A. M. VERSTEEG, S. M. KORTE & D. 
JAARSMA. 1989. Vasopressin and related peptides: Involvement in central cardiovascular 
regulation. In Hypertension, Brain Catecholamines and Peptides. F. P. Nykamp & D. de 
Wied, Eds.: 99- 110. Elsevier. Amsterdam. 
3. TRIBOLLET, E., C. BARBERIS, . JARD, M. DUBOIS-DAUPHIN & J. J. DREIFUSS. 1988. Brain 
Res. 442: 105-118. 
4. ROOZENDAAL, B., G. H. M. SCHOORLEMMER, J. M. KOOLHAAS & B. BOHUS. 1990. 20th 
Annual Meeting of the Society of Neuroscience, St. Louis, MO (USA). Abstr. 221.14. 
5 .  ELANDS, J., C. BARBERIS & S. JARD. 1988. Am. J. Physiol. 254 E31-E38. 


Crossref

University of Groningen

   University of GroningenOpposite effects of central amygdaloid vasopressin and oxytocin on the regulation ofconditioned stress responses in male ratsRoozendaal, B.; Schoorlemmer, G.H.M.; Wiersma, A.; Sluyter, S.; Driscoll, P.; Koolhaas,J.M.; Bohus, B.Published in:Annals of the New York Academy of SciencesDOI:10.1111/j.1749-6632.1992.tb34384.xIMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite fromit. Please check the document version below.Document VersionPublisher's PDF, also known as Version of recordPublication date:1992Link to publication in University of Groningen/UMCG research databaseCitation for published version (APA):Roozendaal, B., Schoorlemmer, G. H. M., Wiersma, A., Sluyter, S., Driscoll, P., Koolhaas, J. M., & Bohus,B. (1992). Opposite effects of central amygdaloid vasopressin and oxytocin on the regulation of conditionedstress responses in male rats. Annals of the New York Academy of Sciences, 652(1), 460-461.https://doi.org/10.1111/j.1749-6632.1992.tb34384.xCopyrightOther than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of theauthor(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).The publication may also be distributed here under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license.More information can be found on the University of Groningen website: https://www.rug.nl/library/open-access/self-archiving-pure/taverne-amendment.Take-down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons thenumber of authors shown on this cover page is limited to 10 maximum.Opposite Effects of Central Amygdaloid Vasopressin and Oxytocin on the Regulation of Conditioned Stress Responses in Male Rats 3. ROOZENDAAL,asb G. H. M. SCHOORLEMMERP A. WIERSMA,' S. SLUYTER," P. DRISCOLL,' J. M. KOOLHAASP AND 3. BOHUS ' 'Department of Animal Physiologv University of Groningen Centre for Behavioural, Cognitive and Neuro-Sciences 9750 AA Haren, the Netherlands 'Behavioral Biologv Laboratory ETHZ CH-8092 Ziirich, Switzerland The central nucleus of the amygdala (CEA) seems to be selectively involved in the passive component of the behavioral (immobility) and the accompanying parasympa- thetic response (bradycardia) during conditioned environmental challenges,' leaving conditioned sympathetic, adreno-medullary, and adreno-cortical responses unaffected (Roozendaal et al., in preparation). Robust monosynaptic, .peptidergic connections to the vagal complex and periaqueductal gray may be mediating these effects. Intracerebroventricularly administered arginine-8-vasopressin (AVP) potentiates these stress-induced bradycardiac and immobility responses.* AVP may exert these effects via AVP and/or oxytocin (OT) receptive systems in the CEA.' Microinfusion of AVP (dissolved in 1 p1 artificial CSF) into the CEA of conscious male Wistar rats under resting conditions, leads to a dose-dependent change in heart rate and behavior: A high dose of AVP (2 ng), but not lower ones (20 and 200 pg), causes a transient increase in heart rate and behavioral a~t iv i ty .~  Moreover, in a part of the animals, the low dose of AVP even causes an opposite effect, that is, bradycardia. OT administration (2 ng) induces responses similar to those induced with the high dose of AVP. Neither plasma norepinephrine nor epinephrine is influenced after AVP infusion, suggesting that the tachycardia is due to reduced parasympathetic control, rather than a stimula- tion of the sympathetic outflow. The effects of AVP and OT during conditioned stress were studied in male Roman high-avoidance (RHA) and Roman low-avoidance. (RLA) rats, selected genetically for shuttle-box acquisition behavior. In RLA rats the cardiac and behavioral responses to the emotional stressor are bradycardia and immobility, 'Address for correspondence: Department of Animal Physiology, University of Groningen, Centre for Behavioural, Cognitive and Neuro-Sciences, P.O. Box 14,9750 AA Haren, the Nether- lands. 460 ROOZENDAAL et al.: REGULATION OF STRESS RESPONSE 461 suggesting an important role of the CEA in these rats. The RHA rats, however, fail to show any change in heart rate or immobility in response to a conditioned stress situation. The low dose of AVP in the CEA of conscious RLA rats causes an enhance- ment of the stress-induced bradycardiac and immobility response. However, the high dose of AVP (2 ng) and OT (200 pg) attenuate the bradycardiac and immobility response in the RLA rats. As expected, infusion of AVP and OT in the RHA rats fails to induce any change in heart rate or immobility. Binding studies revealed that in contrast to the selective and highly potent AVP receptor, the OT receptor recognizes AVP and OT with similar affinit~.~ This suggests that the behavioral and autonomic responses of the high dose of AVP may be caused by OT receptor activation. In conclusion, central amygdaloid vasopressinergic and oxytocinergic receptor- mediated mechanisms seem to exert opposite effects on the regulation of stress-induced autonomic and behavioral responses. AVP enhances the passive components of the conditioned stress response. The oxytocinergic system attenuates the CEA output. The stimulating role of AVP in passive coping may be general and has also been reported in other brain regions. The differences between RLA and RHA males suggest that these peptidergic mechanisms are only active in rats that preferentially adopt a passive coping strategy.2 REFERENCES 1. ROOZENDAAL, B., J. M. KOOLHAAS & B. BOHUS. 1990. Behav. Brain Res. 41: 39-48. 2. BOHUS, B., J. M. KOOLHAAS, P. G. M. LUITEN, C. A. M. VERSTEEG, S. M. KORTE & D. JAARSMA. 1989. Vasopressin and related peptides: Involvement in central cardiovascular regulation. In Hypertension, Brain Catecholamines and Peptides. F. P. Nykamp & D. de Wied, Eds.: 99- 110. Elsevier. Amsterdam. 3. TRIBOLLET, E., C. BARBERIS, . JARD, M. DUBOIS-DAUPHIN & J. J. DREIFUSS. 1988. Brain Res. 442: 105-118. 4. ROOZENDAAL, B., G. H. M. SCHOORLEMMER, J. M. KOOLHAAS & B. BOHUS. 1990. 20th Annual Meeting of the Society of Neuroscience, St. Louis, MO (USA). Abstr. 221.14. 5 .  ELANDS, J., C. BARBERIS & S. JARD. 1988. Am. J. Physiol. 254 E31-E38. 

ARTS repository - University of Groningen

University of GroningenOpposite effects of central amygdaloid vasopressin and oxytocin on the regulation ofconditioned stress responses in male ratsRoozendaal, B.; Schoorlemmer, G.H.M.; Wiersma, A.; Sluyter, S.; Driscoll, P.; Koolhaas,J.M.; Bohus, B.Published in:Annals of the New York Academy of SciencesDOI:10.1111/j.1749-6632.1992.tb34384.xIMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite fromit. Please check the document version below.Document VersionPublisher's PDF, also known as Version of recordPublication date:1992Link to publication in University of Groningen/UMCG research databaseCitation for published version (APA):Roozendaal, B., Schoorlemmer, G. H. M., Wiersma, A., Sluyter, S., Driscoll, P., Koolhaas, J. M., & Bohus,B. (1992). Opposite effects of central amygdaloid vasopressin and oxytocin on the regulation of conditionedstress responses in male rats. Annals of the New York Academy of Sciences, 652(1), 460-461.https://doi.org/10.1111/j.1749-6632.1992.tb34384.xCopyrightOther than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of theauthor(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).The publication may also be distributed here under the terms of Article 25fa of the Dutch Copyright Act, indicated by the “Taverne” license.More information can be found on the University of Groningen website: https://www.rug.nl/library/open-access/self-archiving-pure/taverne-amendment.Take-down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons thenumber of authors shown on this cover page is limited to 10 maximum.Download date: 24-12-2025Opposite Effects of Central Amygdaloid Vasopressin and Oxytocin on the Regulation of Conditioned Stress Responses in Male Rats 3. ROOZENDAAL,asb G. H. M. SCHOORLEMMERP A. WIERSMA,' S. SLUYTER," P. DRISCOLL,' J. M. KOOLHAASP AND 3. BOHUS ' 'Department of Animal Physiologv University of Groningen Centre for Behavioural, Cognitive and Neuro-Sciences 9750 AA Haren, the Netherlands 'Behavioral Biologv Laboratory ETHZ CH-8092 Ziirich, Switzerland The central nucleus of the amygdala (CEA) seems to be selectively involved in the passive component of the behavioral (immobility) and the accompanying parasympa- thetic response (bradycardia) during conditioned environmental challenges,' leaving conditioned sympathetic, adreno-medullary, and adreno-cortical responses unaffected (Roozendaal et al., in preparation). Robust monosynaptic, .peptidergic connections to the vagal complex and periaqueductal gray may be mediating these effects. Intracerebroventricularly administered arginine-8-vasopressin (AVP) potentiates these stress-induced bradycardiac and immobility responses.* AVP may exert these effects via AVP and/or oxytocin (OT) receptive systems in the CEA.' Microinfusion of AVP (dissolved in 1 p1 artificial CSF) into the CEA of conscious male Wistar rats under resting conditions, leads to a dose-dependent change in heart rate and behavior: A high dose of AVP (2 ng), but not lower ones (20 and 200 pg), causes a transient increase in heart rate and behavioral a~t iv i ty .~  Moreover, in a part of the animals, the low dose of AVP even causes an opposite effect, that is, bradycardia. OT administration (2 ng) induces responses similar to those induced with the high dose of AVP. Neither plasma norepinephrine nor epinephrine is influenced after AVP infusion, suggesting that the tachycardia is due to reduced parasympathetic control, rather than a stimula- tion of the sympathetic outflow. The effects of AVP and OT during conditioned stress were studied in male Roman high-avoidance (RHA) and Roman low-avoidance. (RLA) rats, selected genetically for shuttle-box acquisition behavior. In RLA rats the cardiac and behavioral responses to the emotional stressor are bradycardia and immobility, 'Address for correspondence: Department of Animal Physiology, University of Groningen, Centre for Behavioural, Cognitive and Neuro-Sciences, P.O. Box 14,9750 AA Haren, the Nether- lands. 460 ROOZENDAAL et al.: REGULATION OF STRESS RESPONSE 461 suggesting an important role of the CEA in these rats. The RHA rats, however, fail to show any change in heart rate or immobility in response to a conditioned stress situation. The low dose of AVP in the CEA of conscious RLA rats causes an enhance- ment of the stress-induced bradycardiac and immobility response. However, the high dose of AVP (2 ng) and OT (200 pg) attenuate the bradycardiac and immobility response in the RLA rats. As expected, infusion of AVP and OT in the RHA rats fails to induce any change in heart rate or immobility. Binding studies revealed that in contrast to the selective and highly potent AVP receptor, the OT receptor recognizes AVP and OT with similar affinit~.~ This suggests that the behavioral and autonomic responses of the high dose of AVP may be caused by OT receptor activation. In conclusion, central amygdaloid vasopressinergic and oxytocinergic receptor- mediated mechanisms seem to exert opposite effects on the regulation of stress-induced autonomic and behavioral responses. AVP enhances the passive components of the conditioned stress response. The oxytocinergic system attenuates the CEA output. The stimulating role of AVP in passive coping may be general and has also been reported in other brain regions. The differences between RLA and RHA males suggest that these peptidergic mechanisms are only active in rats that preferentially adopt a passive coping strategy.2 REFERENCES 1. ROOZENDAAL, B., J. M. KOOLHAAS & B. BOHUS. 1990. Behav. Brain Res. 41: 39-48. 2. BOHUS, B., J. M. KOOLHAAS, P. G. M. LUITEN, C. A. M. VERSTEEG, S. M. KORTE & D. JAARSMA. 1989. Vasopressin and related peptides: Involvement in central cardiovascular regulation. In Hypertension, Brain Catecholamines and Peptides. F. P. Nykamp & D. de Wied, Eds.: 99- 110. Elsevier. Amsterdam. 3. TRIBOLLET, E., C. BARBERIS, . JARD, M. DUBOIS-DAUPHIN & J. J. DREIFUSS. 1988. Brain Res. 442: 105-118. 4. ROOZENDAAL, B., G. H. M. SCHOORLEMMER, J. M. KOOLHAAS & B. BOHUS. 1990. 20th Annual Meeting of the Society of Neuroscience, St. Louis, MO (USA). Abstr. 221.14. 5 .  ELANDS, J., C. BARBERIS & S. JARD. 1988. Am. J. Physiol. 254 E31-E38. 

   University of GroningenOpposite Effects of Central Amygdaloid Vasopressin and Oxytocin on the Regulation ofConditioned Stress Responses in Male RatsRoozendaal, B.; Schoorlemmer, G.H.M.; Wiersma, A.; Sluyter, S.; Driscoll, P.; Koolhaas,J.M.; Bohus, B.Published in:Annals of the New York Academy of SciencesDOI:10.1111/j.1749-6632.1992.tb34384.xIMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite fromit. Please check the document version below.Document VersionPublisher's PDF, also known as Version of recordPublication date:1992Link to publication in University of Groningen/UMCG research databaseCitation for published version (APA):Roozendaal, B., Schoorlemmer, G. H. M., Wiersma, A., Sluyter, S., Driscoll, P., Koolhaas, J. M., & Bohus,B. (1992). Opposite Effects of Central Amygdaloid Vasopressin and Oxytocin on the Regulation ofConditioned Stress Responses in Male Rats. Annals of the New York Academy of Sciences, 652(1), 460-461. https://doi.org/10.1111/j.1749-6632.1992.tb34384.xCopyrightOther than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of theauthor(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).Take-down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons thenumber of authors shown on this cover page is limited to 10 maximum.Download date: 12-11-2019Opposite Effects of Central Amygdaloid Vasopressin and Oxytocin on the Regulation of Conditioned Stress Responses in Male Rats 3. ROOZENDAAL,asb G. H. M. SCHOORLEMMERP A. WIERSMA,' S. SLUYTER," P. DRISCOLL,' J. M. KOOLHAASP AND 3. BOHUS ' 'Department of Animal Physiologv University of Groningen Centre for Behavioural, Cognitive and Neuro-Sciences 9750 AA Haren, the Netherlands 'Behavioral Biologv Laboratory ETHZ CH-8092 Ziirich, Switzerland The central nucleus of the amygdala (CEA) seems to be selectively involved in the passive component of the behavioral (immobility) and the accompanying parasympa- thetic response (bradycardia) during conditioned environmental challenges,' leaving conditioned sympathetic, adreno-medullary, and adreno-cortical responses unaffected (Roozendaal et al., in preparation). Robust monosynaptic, .peptidergic connections to the vagal complex and periaqueductal gray may be mediating these effects. Intracerebroventricularly administered arginine-8-vasopressin (AVP) potentiates these stress-induced bradycardiac and immobility responses.* AVP may exert these effects via AVP and/or oxytocin (OT) receptive systems in the CEA.' Microinfusion of AVP (dissolved in 1 p1 artificial CSF) into the CEA of conscious male Wistar rats under resting conditions, leads to a dose-dependent change in heart rate and behavior: A high dose of AVP (2 ng), but not lower ones (20 and 200 pg), causes a transient increase in heart rate and behavioral a~t iv i ty .~  Moreover, in a part of the animals, the low dose of AVP even causes an opposite effect, that is, bradycardia. OT administration (2 ng) induces responses similar to those induced with the high dose of AVP. Neither plasma norepinephrine nor epinephrine is influenced after AVP infusion, suggesting that the tachycardia is due to reduced parasympathetic control, rather than a stimula- tion of the sympathetic outflow. The effects of AVP and OT during conditioned stress were studied in male Roman high-avoidance (RHA) and Roman low-avoidance. (RLA) rats, selected genetically for shuttle-box acquisition behavior. In RLA rats the cardiac and behavioral responses to the emotional stressor are bradycardia and immobility, 'Address for correspondence: Department of Animal Physiology, University of Groningen, Centre for Behavioural, Cognitive and Neuro-Sciences, P.O. Box 14,9750 AA Haren, the Nether- lands. 460 ROOZENDAAL et al.: REGULATION OF STRESS RESPONSE 461 suggesting an important role of the CEA in these rats. The RHA rats, however, fail to show any change in heart rate or immobility in response to a conditioned stress situation. The low dose of AVP in the CEA of conscious RLA rats causes an enhance- ment of the stress-induced bradycardiac and immobility response. However, the high dose of AVP (2 ng) and OT (200 pg) attenuate the bradycardiac and immobility response in the RLA rats. As expected, infusion of AVP and OT in the RHA rats fails to induce any change in heart rate or immobility. Binding studies revealed that in contrast to the selective and highly potent AVP receptor, the OT receptor recognizes AVP and OT with similar affinit~.~ This suggests that the behavioral and autonomic responses of the high dose of AVP may be caused by OT receptor activation. In conclusion, central amygdaloid vasopressinergic and oxytocinergic receptor- mediated mechanisms seem to exert opposite effects on the regulation of stress-induced autonomic and behavioral responses. AVP enhances the passive components of the conditioned stress response. The oxytocinergic system attenuates the CEA output. The stimulating role of AVP in passive coping may be general and has also been reported in other brain regions. The differences between RLA and RHA males suggest that these peptidergic mechanisms are only active in rats that preferentially adopt a passive coping strategy.2 REFERENCES 1. ROOZENDAAL, B., J. M. KOOLHAAS & B. BOHUS. 1990. Behav. Brain Res. 41: 39-48. 2. BOHUS, B., J. M. KOOLHAAS, P. G. M. LUITEN, C. A. M. VERSTEEG, S. M. KORTE & D. JAARSMA. 1989. Vasopressin and related peptides: Involvement in central cardiovascular regulation. In Hypertension, Brain Catecholamines and Peptides. F. P. Nykamp & D. de Wied, Eds.: 99- 110. Elsevier. Amsterdam. 3. TRIBOLLET, E., C. BARBERIS, . JARD, M. DUBOIS-DAUPHIN & J. J. DREIFUSS. 1988. Brain Res. 442: 105-118. 4. ROOZENDAAL, B., G. H. M. SCHOORLEMMER, J. M. KOOLHAAS & B. BOHUS. 1990. 20th Annual Meeting of the Society of Neuroscience, St. Louis, MO (USA). Abstr. 221.14. 5 .  ELANDS, J., C. BARBERIS & S. JARD. 1988. Am. J. Physiol. 254 E31-E38. 

Opposite Effects of Central Amygdaloid Vasopressin and Oxytocin on the Regulation of Conditioned Stress Responses in Male Rats

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