An outbreak of viral encephalitis occurred in northern India in 2006. Attempts to identify an etiologic agent in cerebrospinal fluid by using reverse transcription–PCR showed positivity to enterovirus (EV) in 66 (21.6%) of 306 patients. Sequencing and phylogenetic analyses of PCR products from 59 (89.3%) of 66 specimens showed similarity with EV-89 and EV-76 sequences

Gajanan N. Sapkal

Vijay P. Bondre

Pradip V. Fulmali

Pooja Patil

Vipul Dadhania

Vijay M. Ayachit

Daya Gangale

K.P. Kushwaha

A.K. Rathi

Shobha D. Chitambar

Akhilesh Chandra Mishra

Milind M. Gore

Kennedy

Sapkal

Zoll

Puig

Oberste

Kumar

Wildin

Hayward

Kehle

Kapoor

English

PubMed

Using rapid amplification of cDNA ends (RACE), we have cloned the 5'-untranslated region (5'-UTR) of the N-methyl-D-aspartate receptor subunit epsilon(2) from murine forebrain-derived mRNA. We identified two distinct types of cDNA species differing in the presence or absence of one exon sequence. Sequencing of the 5'-non-coding region of the epsilon(2) gene revealed that the epsilon(2) 5'-UTR consists of three untranslated exons located at least 20 kb upstream of exon 4 that contains the ATG codon for initiation of translation. This genomic organization shows a close similarity to the epsilon(3) gene. The transcriptional start site was determined by primer extension assays. Expression of the alternative exon sequence was shown by in situ hybridization in the murine brain. Basal transcriptional activity of the epsilon(2) promoter was detected in different neuronal and non-neuronal cell lines with transient reporter gene expression assays. Potential SP1 and CREB binding sites were found in the promoter region. Specific binding of these transcription factors was demonstrated in electrophoretic mobility shift assays. (C) 1998 Elsevier Science B.V.</p

Klein, Matthias

Pieri, Isabelle

Uhlmann, Frank

Pfizenmaier, Klaus

Eisel, Ulrich

Dissertations of the University of Groningen

Cloning and characterization of promoter and 5 '-UTR of the NMDA receptor subunit epsilon(2):evidence for alternative splicing of 5 '-non-coding exon

University of Groningen

Proceedings - University of Groningen

University of Groningen Research Database

  
 University of Groningen
Cloning and characterization of promoter and 5'-UTR of the NMDA receptor subunit ε2
Klein, Matthias; Pieri, Isabelle; Uhlmann, Frank; Pfizenmaier, Klaus; Eisel, Ulrich
Published in:
Gene
DOI:
10.1016/S0378-1119(98)00005-5
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Publication date:
1998
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Klein, M., Pieri, I., Uhlmann, F., Pfizenmaier, K., & Eisel, U. (1998). Cloning and characterization of
promoter and 5'-UTR of the NMDA receptor subunit ε2: evidence for alternative splicing of 5'-non-coding
exon. Gene, 208(2), 259-269. DOI: 10.1016/S0378-1119(98)00005-5
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Cloning and characterization of promoter and 5'-UTR of the NMDA receptor subunit ε2:evidence for alternative splicing of 5'-non-coding exon

ARTS repository - University of Groningen

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