Aksnes

Arnesen

Bedell

Behnia

Bienvenut

Caesar

Ciechanover

Esmailpour

Forte

Foyn

Gautschi

Goetze

Hershko

Hwang

Håvard Foyn

Ingram

Jörnvall

Kalvik

Kari E. Fladmark

Langheinrich

Line M. Myklebust

Liszczak

Mattila

Miller

Mullen

Myklebust

Park

Peterson

Polevoda

Pollard

Puja Thiel

Rasmus Ree

Robu

Rope

Scott

Shemorry

Shin

Starheim

Sönnichsen

Thomas Arnesen

Thompson

Van Damme

Wang

Williams

Yoon

Bioscience Reports

N-terminal acetylation, catalysed by N-terminal acetyltransferases (NATs), is among the most common protein modifications in eukaryotes and involves the transfer of an acetyl group from acetyl-CoA to the α-amino group of the first amino acid. Functions of N-terminal acetylation include protein degradation and sub-cellular targeting. Recent findings in humans indicate that a dysfunctional Nα-acetyltransferase (Naa) 10, the catalytic subunit of NatA, the major NAT, is associated with lethality during infancy. In the present study, we identified the Danio rerio orthologue zebrafish Naa 10 (zNaa10). In vitro N-terminal acetylation assays revealed that zNaa10 has NAT activity with substrate specificity highly similar to that of human Naa10. Spatiotemporal expression pattern was determined by in situ hybridization, showing ubiquitous expression with especially strong staining in brain and eye. By morpholino-mediated knockdown, we demonstrated that naa10 morphants displayed increased lethality, growth retardation and developmental abnormalities like bent axis, abnormal eyes and bent tails. In conclusion, we identified the zebrafish Naa10 orthologue and revealed that it is essential for normal development and viability of zebrafish.</jats:p

Crossref

The N-terminal acetyltransferase Naa10 is essential for zebrafish development

http://www.bioscirep.org/content/ppbioscirep/35/5/e00249.full.pdf

The N-terminal acetyltransferase Naa10 is essential for zebrafish development

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