Effects of magnetic bead-based enrichment of NB cells on their gene expression.

Abstract

<p>qPCR arrays were used to analyze the effects of magnetic bead-based enrichment on the expression of 71 genes in NB cells. In (a) the altered gene expression is shown for cells that have been enriched only once after density gradient separation, whereas in (b) the effect of two following magnetic bead-based enrichment steps is shown. Red dots represent genes that are significantly changed (p<0.05, |log₂FC|>1) at given conditions compared to the baseline (LAN-1 cells before spiking into PB). The expression of genes with |log₂FC|>1 but p>0.05 are not considered as significant, as their expression was not coherently changed in the different biological replicates. The log₂ fold change is indicated on the y-axis and the mean Ct values in the x-axis.</p