Application of triarylborane system in selective readout of nucleic acids and live cell fluorescence imaging

Abstract

Detekcijske metode se baziraju na fluorescencijskim biljezima koji se koriste za obilježavanje proteina, određenih DNA/RNA sekvenci i sl. Oni se na DNA/RNA sekvence mogu vezati u utor, interkalirati ili ostvarivati elektrostatske interakcije. U ovom istraživanju spektroskopski i kalorimetrijski su okarakterizirane interakcije dva triarilboranska derivata SG224 i SG216 s nizom jednolančanih i dvolančanih polinukleotida. Spoj SG216 se ne veže na polinukleotide. Spoj SG224 se veže na DNA/RNA strukture kombinacijom više različitih načina te nije selektivn prema jednolančanim ili dvolančanim polinukleotidima ni prema DNA ili RNA uzvojnicama. Istovremeno s procesom vezanja odvija se i neki drugi proces, vjerojatno agregacija spoja, te se zbog toga ne može preciznije odrediti način vezanja. Pretpostavlja se da je vezanje u utor najznačajniji način vezanja, što potvrđuje i činjenica da SG224 ne pokazuje afinitet k vezanju p(dGdC)2 niza, čiji je utor sterički blokiran. Spoj se može koristiti kao spektropolarimetrijski biljeg za pdA sekvence, jer se prilikom vezanja spoja na pdA sekvence inducira jaki CD signal na 402 nm. Spoj nema značajan antiproliferativan učinak.Detection methods are based on fluorescence markers which are used for marking proteins, certain DNA/RNA sequences etc. They bind into the groove, intercalate or achive electrostatic interactions with DNA/RNA sequences. In this research we spectroscopically and calorimaterically characterize interactions between two three-aryl-borane derivatives SG224 and SG216 with series of single - and double-stranded polynucleotides. Compound SG216 does not bind to the polynucleotides. Compound SG224 binds to DNA/RNA structures in several different patterns. However, it´s not selective for neither single- nor double-stranded polynucleotides or to DNA/RNA helixes. Simultaneously with the binding process, another process also occurs – most likely aggregation of the compound. Therefore the binding method cannot be precisely defined. It´s however assumed that binding into the groove is most likely as it was confirmed by the fact SG224 does not show affinity to bind p(dGdC)2 string, whose groove is sterically blocked. Compound can be used as spectro-polarimetric marker for pdA sequences, due to big CD signal on 402 nm that is induced when SG224 binds pdA sequences. The compound does not have significant antiproliferative effect