Papp, Beata

Petrović, Bojan

Petrović, Nevena

Sabovljević, Aneta

Sabovljević, Marko

Szurdoki, Erzsebet

Vujičić, Milorad

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2013. szeptember 30. – október1. Eger57INSIGHTS INTO BIOLOGY OF THE MOSS ENTOSTHODON HUNGARICUSMarkoSabovljević1*,MiloradVujičić1, Beata Papp2, Nevena Petrović1, Bojan Petrović1, Erzsebet Szurdoki2,AnetaSabovljević11Institute of Botany and Garden, Faculty of Biology, University of Belgrade, Takovska 43, 11000 Belgrade, Serbia2Department of Botany, Hungarian Natural History Museum H–1476 Budapest, Pf. 222, Hungary*marko@bio.bg.ac.rsAbsztrakt:The moss Entosthodon hungaricus (Boros) Loeske is an funaroid moss spe-cies with scattered distribution and interesting ecology. It is considered as a fac-ultative moss halophyte that inhabits the salty environment in central, eastern and southern Europe. It has a quick life span. Due to specific ecology, it is an endangered species in European level. For the purpose of the investigation of the biological features of this species, we have established in vitro culture and stud-ied the feature of this moss. The best micropropagation and gametophyte devel-opment were achieved on BCD medium supplemented with 0.1μM IBA and0.03μMBAbased onmultiplication index in vitro.The highest production ofsecondary protonema was achieved on BCD medium enriched with0.1μMIBAand0.3μMBA.Also, the apogamous sporophytes of the moss Entosthodon hungaricus were reported for the first time. The plants under lower light intensity and constant water accessibility produced apogamous sporophytes under controlled condi-tions. We can assume the biological significance of such a fenomena, as the advantage for reproduction in short and quick life span in case that some other plants overgrown it and constrain the shortly available auspicious resources.The in vitro exposure of E. hungaricus gametophytes to NaCl stress showed this species to be salt tolerant. Comparison of E. hungaricus with its relative moss Physcomitrella patens (both Funariacerae, with shuttle survival strategies) in the features as survival, index of multiplications (i.e. new shoots produced), total chlorophyll content in both species showed almost linearly decrease with NaCl concentration addition to the growth medium. In contrast, carotenoid con-tent increased. The chlorophyll a/b ratio decreased in both species, E. hungaricus and P. patens. Catalases in E. hungaricus abruptly increase activity with low salt stress, and then slightly decrease by each further increase of salt stress. The ac-Poszterszekció58tivity of peroxydases, after slight decrease in low salt stress conditions, increased with the higher salt concentration up to 300mM NaCl. With further salt increase, their activity significantly falled down. Catalase activity in P. patens show no clear pattern, while the activity of peroxidases abruptly decreased with salt stress increased. Superoxyddismutase had more activated izoformes in E. hungaricus than in P. patens during the salt stress, which implicate that enzymes of anti-oxidative system react to salt stress and take part in biology of tolerance to NaCl in mosses, as a first level response. All these parameters showed E. hungaricus to be more stress tolerant moss than P. patens.The moss E. hungaricus, a part for deserving special attention to its biology due to its conservation values, express the interesting and unique characteristics among bryophytes and plants in general, which can elucidate many brain teaser of plant life or be used in biotechnology. 

Insights into biology of the moss Entosthodon hungaricus [Poszter absztrakt]

http://publikacio.uni-eszterhazy.hu/2971/1/57-58_Marko.pdf

Insights into biology of the moss Entosthodon hungaricus [Poszter absztrakt]

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