F. Acocella

F. Di Cesare

G. Ravasio

P. Cagnardi

R. Villa

AIR Universita degli studi di Milano

Proceeding of Veterinary and Animal Science Days 2016, 8th- 10th June, Milan, Italy 
HAF © 2013 
Vol. III, No. 1s  ISSN: 2283-3927 
   
 
l 
 
 
 
 
 
 
 
 
 
 
 
 
Keywords 
Cefazolin, dog, pharmacokinetic, 
pharmacodynamics 
 
CORRESPONDING AUTHOR 
Di Cesare Federica 
federica.dicesare@unimi.it   
 
JOURNAL HOME PAGE 
riviste.unimi.it/index.php/haf 
Cefazolin in dog: preliminary results for 
pharmacokinetic and pharmacodynamic 
parameters. 
 
F. Di Cesarea*, P. Cagnardia, G. Ravasiob, F. Acocellaa, R. Villaa 
 
 
aDept. Health, Animal Science and Food Safety, Università degli Studi di Milano, 
Italy; 
bDept Veterinary Medicine, Università degli Studi di Milano, Italy 
 
Abstract 
Cefazolin, a first generation cephalosporin, is commonly used in small animal surgery to avoid post-
operative infections. The aims of the study were 1) assess cefazolin pharmacokinetics (PK) in dogs 
undergoing gonadectomy, 2) correlate PK and pharmacodynamics (PD) parameters and 3) attest 
the efficacy of dosage regimen through a PK/PD approach. Thirty minutes before surgery, 25 mg/kg 
of cefazolin were administrated intravenously (IV) to 9 dogs (weight 22±7.5 kg; age 1.3±0.7 years). 
Blood samples were taken at prefixed times from 0 to 8 h. Quantification of cefazolin 
concentrations was performed through a validate HPLC method with UV detection (Kunicki, 2012). 
A two-compartmental model best described the PK profile of cefazolin. Literature MIC50 against 
canine Pasteurella spp., Staphylococcus spp. and Streptococcus spp. ranged from 0.25 to 0.5 µg/mL 
(Goldstein, 2012). Distribution and elimination half-lives were 0.3 ± 0.2 h and 3 ± 1.6 h, respectively; 
area under the curve was 182.3±50.6 h*µg/mL; volume of distribution and clearance at steady state 
were 383.5±58.1 mL/kg and 150.2±49.8 mL/h/kg, respectively. The PK/PD index for cephalosporins 
efficacy is time above MIC (T>MIC) for 60% of dosing interval with values 4 times higher the MIC50. 
Our values of T>MIC, calculated with MIC 0.5 mg/mL, was for 100 % of the observation period from 
2 to 6 times higher than MIC. Preliminary results showed a good efficacy of cefazolin against all the 
bacterial strains evaluated. Therefore, a dosage regimen of 25 mg/kg IV every 8h might represent a 
valid tool in order to prevent surgical infections in small animal practice. 
 
References 
Goldstein, E.J., Citron, D.M., Merriam, C.V., Tyrrell, K.L., 2012. Ceftaroline versus isolates from animal bite wounds: 
comparative in vitro activities against 243 isolates, including 156 Pasteurella species isolates. Antimicrobial Agents and 
Chemotherapy 56, 6319–6323. 
Kunicki, P.K., Wàs J., 2012. Simple HPLC method for cefazolin determination in serum - validation and stability testing. Journal 
of Chromatography B 911, 133-139. 


Cefazolin in dog : preliminary results for pharmacokinetic and pharmacodynamic parameters

https://air.unimi.it/retrieve/handle/2434/527711/915573/CefazolinDog_2016.pdf

Cefazolin in dog : preliminary results for pharmacokinetic and pharmacodynamic parameters

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