Role of Endogenous NGF in Modulation of Neuro-glial Network in the Spinal Cord of a Rat Model of Neuropathic Pain

Abstract

Interaction between glial and neuronal cells seems to be necessary for the correct neuronal function and modifications of neuro-glial network strongly contribute to several neurodegenerative processes. Based on NGF activity on DRG and sensory neurons in the spinal cord, we have used the Spared Nerve Injury (SNI) rat model to investigate the role of endogenous NGF in maintaining homeostatic synaptic conditions. We found that restoring the balance of NGF maturation and degradation reduces neuropathic behaviour by reverting the alterations of neuronal and glial components. In addiction, by using neuronally differentiated PC12 cell, we identify a novel mechanism of neuroprotection by NGF that involves modulation of mitochondrial function and autophagy