A. Chetta

A. Sala

C. Buccellati

D. Olivieri

E. Clini

F. Fumagalli

G. Ciabattoni

G. Folco

M. Aiello

M. Losi

P. Montuschi

R. Pisi

R. Shohreh

AIR Universita degli studi di Milano

 / Thematic Poster Session / Monday, May 17/8:15B37 CHRONIC OBSTRUCTIVE PULMONARY DISEASE PATHOGENESIS IAM-4:00 PM / Area D, Hall G (First Level), Morial Convention CenterBiomarkers Of Inflammation And Oxidative Stress In Patients With COPD And Healthy Smokers, M. Aiello , C. Buccellati , G. Ciabattoni , A. Chetta , E. Clini , G. Folco , F. Fumagalli , M. Losi , D. Olivieri , R. PisiP. Montuschi1 2 3 4 2 5 3 3 5 2, R. Shohreh , A. Sala2 4 3Catholic University of the Sacred Heart, Roma, Italy, University of Parma, Parma, Italy, University of Milan, Milan, Italy, University of1 2 3 4Chieti, Chieti, Italy, University of Modena, Modena, Italy5Corresponding author's email: pmontuschi@rm.unicatt.itWe measured urinary 8-isoprostane (IsoP), a biomarker of oxidative stress, and fractional exhaled nitric oxide (FeNO), a biomarker of airwayinflammation, in 8 patients with COPD who were current smokers (COPD CS) (7/1 males/females, age 60±4 yrs, mean±SEM, FEV165.8±4.8% pred), 9 patients with COPD who were ex-smokers (COPD ES) (7/2 males/females, age 66±3 yrs, FEV  50.6±3.6% pred), and 101healthy smokers (HS) (9/1 m/f, 43±4 yrs, FEV  101.7±3.1% pred). We also measured prostaglandin (PG)E2, that can have pro- and1anti-inflammatory effects, in sputum supernatants in 6 COPD CS (5/1 males/females, age 60±5 yrs, FEV  62.9±4.4% pred), 12 COPD ES1(10/2 males/females, age 67±2 yrs, FEV  51.3±3.3% pred), and 4 HS (3/1 males/females, age 43±6 yrs, FEV  98.3±4.8% pred). Compared1 1with COPD ES (268.0±28.8 pg/mg creatinine), urinary 8-IsoP was increased in COPD CS (506.1±85.6 pg/mg creatinine, p<0.05), but not inHS (385.9±52.2 pg/mg creatinine, p=ns), reflecting the higher degree of oxidative stress in COPD CS. FeNO was increased in COPD ES(24.8±6.0 ppb) compared with COPD CS (9.6±4.2 ppb, p<0.04) and HS (8.7±1.5 ppb, p<0.04) likely reflecting the inhibitory effect ofsmoking on endogenous NO production and/or increased NO breakdown due to free radicals. Compared with COPD ES (138.9±22.3pg/ml), sputum PGE  was increased in COPD CS (308.0±96.6 pg/ml, p<0.04) and HS (385.5±134.6, p<0.01) indicating a stimulating effect of2smoking itself on cyclo-oxygenase activity. Different biomarkers reflect different aspects of inflammation and/or oxidative stress inpatients with COPD and healthy smokers.This abstract is funded by: PRIN-Ministry of University and ResearchAm J Respir Crit Care Med 181;2010:A2892Internet address: www.atsjournals.org Online Abstracts Issue

Biomarkers of inflammation and oxidative stress in patients with COPD and healthy smokers

https://air.unimi.it/retrieve/handle/2434/206755/237331/AJRCCM2010.pdf

Biomarkers of inflammation and oxidative stress in patients with COPD and healthy smokers

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