http://www.godac.jamstec.go.jp/darwin/cruise/natsushima/nt06-04/ehttp://www.godac.jamstec.go.jp/darwin/cruise/natsushima/nt06-05/ehttp://www.godac.jamstec.go.jp/darwin/cruise/natsushima/nt06-22/ehttp://www.godac.jamstec.go.jp/darwin/cruise/natsushima/nt08-02/ehttp://www.godac.jamstec.go.jp/darwin/cruise/chikyu/902/

CHIKARAISHI, Yoshito

Hinrichs, Kai-Uwe

HINRICHS, Kai-Uwe

INAGAKI, Fumio

KITAZATO, Hiroshi

MORONO, Yuki

NOMAKI, Hidetaka

OGAWA, Nanako

OHKOUCHI, Naohiko

TAKANO, Yoshinori

力石, 嘉人

北里, 洋

大河内, 直彦

小川, 奈々子

稲垣, 史生

諸野, 祐樹

野牧, 秀隆

高野, 淑識

English

Deep-sea sediments harbour a vast biosphere. Archaea—one of the three domains of life1—are prevalent in marine environments2, 3, 4, 5, and comprise a significant fraction of the biomass in marine sediments6. Archaeal membranes are well characterized, and are comprised of a glycerol backbone and a nonpolar isoprenoid chain. However, the ecology of sedimentary archaea remains elusive, because it is difficult to grow them in the laboratory. Here, we trace the fate of 13C-labelled glucose added to marine sediments in Sagami Bay, Japan, to determine the in situ mechanisms of membrane synthesis. Following the addition of labelled glucose to sediment samples collected in the region, we placed the cores on the sea floor and sampled them after 9 and 405 days. We found that the 13C was incorporated into the glycerol backbone of archaeal membranes; 13C was apparent after 9 days of incubation, but most pronounced after 405 days. However, the isoprenoid chain of the membranes remained unlabelled. On the basis of the differential uptake of 13C, we suggest that the glycerol unit is synthesized de novo, whereas the isoprenoid unit is synthesized from relic archaeal membranes and detritus, because of the prevalence of these compounds in marine sediments. We therefore suggest that some benthic archaea build their membranes by recycling sedimentary organic compounds

Takano, Yoshinori

Chikaraishi, Yoshito

Ogawa, Nanako O.

Nomaki, Hidetaka

Morono, Yuki

Inagaki, Fumio

Kitazato, Hiroshi

Ohkouchi, Naohiko

Hokkaido University Collection of Scholarly and Academic Papers

Sedimentary membrane lipids recycled by deep-sea benthic archaea

JAMSTEC Repository

Yoshinori Takano

Yoshito Chikaraishi

Nanako O. Ogawa

Hidetaka Nomaki

Yuki Morono

Fumio Inagaki

Hiroshi Kitazato

Kai-Uwe Hinrichs

Naohiko Ohkouchi

Crossref

Nature Geoscience

https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/45753/2/Takano_naturegeosci_SI_2010.pdf

Sedimentary membrane lipids recycled by deep-sea benthic archaea

Abstract

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Hokkaido University Collection of Scholarly and Academic Papers

JAMSTEC Repository

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