To define specific reactivities of PC-BL (WHO/EORTC 2008 classification), we
analyzed, by using an immunohistochemical method, the mAbs of the FACS panel
of the 9th HLDA Workshop on tissue sections of the different cutaneous lymphomas
entities: follicular centre B-cell lymphomas (FCL), marginal zone lymphomas (MZL)
and diffuse large B-cells lymphomas (DLBL) leg-type. Cryostat sections of frozen
tissues were dried for 12 hours, fixed 10" in aceton and stained by an avidin-biotin
immunoalkaline phosphatase method (DAKO). Tissue reactivities of lymphocytes and/or other resident cells were detected by using all mAbs, except for n\ub0 49-51-81-90-91-109. Specific lymphoma reactivities were detected on PC-BL: Group 1 mAbs stained all PC-BL: n\ub0 88 (CD48). Group 2 mAbs stained PC-FCL: n\ub020(hFCRL1); 34(CD152); 35 (CD80), 36(CD126); 37(PD1-CD279); 38(B7-DC-CD273); 40 (CD200); 41(CD130); 46 (CD184-CXCR4); 47 (BTLA-CD272); 50(CD185-CXCR5); 56(CMKLR1); 57 (DR5); 77(Siglec-5/14); 83 (CRTAM); 86(CD229); 89 (CD84); 93(NTBA); 94(HVEM); 96(TWEAK); 97(TCL1); 100(LT-betaR); 106(CD150). Group 3
mAbs stained PC-MZL: n\ub0 36(CD126); 47(BTLA); 50(CXCR5); 55(DR4); 77(Siglec-5/14); 83(CRTAM); 84(DCIR/CLEC4A); 86(CD229); 87(CD319); 93(NTBA). Group 4 mAbs stained PC-DLBL: n\ub0 35(CD80); 36(CD126); 39(CD124); 50(CD185); 55(DR4); 56(CMKLR1); 57(DR5); 75(Dectin-1); 76-101(TREM-1); 82(Integrin
alfaVbeta5); 83(CRTAM); 85-99(GITR); 86(CD229); 87(CD319); 89(CD84); 92(Dr3-
TRAMP); 93(NTBA); 94(HVEM-CD258); 97(TCL-1); 100(Lymphotoxin betaR).
Furthermore, variable mixed reactivities with infiltrating or neoplastic cells were
observed by using several mAbs and other Mabs showed many tissue cross
reactivities and were not easy to evaluate by IHC (further investigations may be done
on "pure" infiltrates, cell suspensions of neoplastic cells or by using double labeling
techniques-confocal laser microscopy). Finally, mAbs n\ub0 21-22-23-30-31-32-33-42-44-45-48-49-51-52-53-54-58-74-78-79-80-81-82-84-90-91-98-109 were negative in all lymphoma cases. This study may be useful to better define specific profile of different PC-BL entities
