Effect of adenosine derivatives on in vitro thrombus formation induced by shear stress

Abstract

Background and Objective : shear-stress is considered to be the first event of platelet aggregation in vivo and platelet adhesion may be enhanced under pathologic conditions (e.g. arterial occlusion) Design and Methods : we wanted to test the effect of adenosine derivatives on platelet aggregation induced by shear-stress. By increasing platelet cAMP adenosine derivatives inhibit platelet activation. This in turn leads to P-selectin (CD62P) exposure, which is known to play a fundamental role in the binding of platelets to leukocytes. This gives rise to thrombus formation Results : the levels of cAMP (pmol/mL) prior to and after treating blood with the following compounds were respectively: PGE1 4.67\ub10.29, 9.33\ub10.58; SP64 5.2\ub10.34, 6.83\ub10.52; 2-Cl-adenosine 5.83\ub1 0.58, 7.45\ub10.55; NECA 7.00\ub12.29, 8.00\ub11.76 Interpretation and Conclusions : high shear rate was studied using a filteraggregometer which could be a good test for analyzing what happens under physiologic conditions compared to other systems in which platelet aggregation only occurs after adding aggregating agents (Born aggregometer