Gas- chromatographic determination of pig plasma malondialdehyde with negative-ion chemical ionization tandem mass spectrometry detection

Abstract

Malondialdehyde (MDA) was estimated in pig plasma by gas chromatography with NICI-MS-MS detection after derivatization with pentafluorobenzyl hydroxylamine. Glutaraldehyde was used as an internal standard. MDA gave two peaks, only the major one was considered for quantitative analysis. In multiple reaction monitoring mode the minimum detectable amount was 40 pmoles per sample. In 9 pig plasma samples, collected at abattoir, the MDA contents found were between 220-1015 pmoles/ml