The purpose of this study is to determine the normal perfusion dynamics in the canine liver using sulphur hexafluoride (Sonovue\uae) as contrast medium. Its effects have been investigated measuring the changes in liver grayscale enhancement in 11 dogs. Each dog received two separate bolus contrast injection (0.03 ml/kg and 0.06 ml/kg). Two different setup of the ultrasound machine were considered. Time/Mean Pixel Value curves were generated for selected regions of the liver, at 2 and at 4 cm of depth. The influence of cardiac output, heart rate and respiratory rate upon the arterial phase time, portal phase time, equilibrium phase, time to peak, time to 50% peak and maximum pixel value were investigate
