Monitoring of omeprazole synthesis by hyphenated LC-SPE/NMR system. NMR and high-school chemical syllabus.

Abstract

U ovom diplomskom radu opisano je praćenje reakcije oksidacije omeprazol sulfida, poslijednjeg koraka prilikom sinteze omeprazola, pomoću spregnutog sustava tekućinska kromatografija-ekstrakcija na čvrstoj fazi-nuklearna magnetska rezonancija (LC-SPE/NMR). Omeprazol je lijek koji se koristi za liječenje bolesti uzrokovane pretjeranim lučenjem želučane kiseline, a djeluje tako da regulira enzimski sustav odgovoran za njezino izlučivanje. U radu su optimirani uvjeti tekućinskokromatografskog odjeljivanja, skladištenja uzoraka na sustavu SPE te snimanja spektara NMR. Uspoređene su metodologije on-line i off-line za praćenje sinteze omeprazola i identifikaciju produkata, nusprodukata i nečistoća. U okviru metodičkog dijela rada prikazan je prijedlog nastavnog sata pod nazivom „Nuklearna magnetska rezonancija“ u kojem je opisana priprema sata namijenjena učenicima četvrtog razreda srednje škole te je detaljno razrađen radni listić za učenike.This thesis describes monitoring the oxidation reaction of omeprazole sulfide, which is the last step in omeprazole synthesis by hyphenated liquid chromatography-solid phase extraction-nuclear magnetic resonance methodology (LC-SPE/NMR). Drug Omeprazole blocks enzyme system responsible for the secretion of gastric acid. That is the reason why it is used for the treatment of diseases caused by hypersecretion of gastric acid. Conditions of liquid chromatography separation, trapping on SPE cartridges and recording of NMR spectra were optimized. On-line and off-line methodologies for monitoring the omeprazole synthesis and identification of products, byproducts and impurities were disscused. In the second part of this thesis one of the possible modern approaches in teaching chemistry for high school students have been elaborated. The title of the topic is „Nuclear magnetic resonance“ and a worksheet for students is enclosed