Akane Furuta

Fumio Arisaka

George B. Witman

Jiro Usukura

Ken-ichi Wakabayashi

King

Mikito Owa

Ritsu Kamiya

Stephen M. King

English

Significance
          In eukaryotic cilia and flagella, outer arm dyneins (OADs) are bound to axonemal doublet microtubules every 24 nm; however, how this regular arrangement is produced remains unknown. To approach this problem, we studied the properties of the OAD-docking complex (ODA-DC), a three-subunit complex that functions as the OAD-docking site on the doublet. Using recombinant ODA-DC, we found that the ODA-DC has an ∼24-nm-long ellipsoidal shape and cooperatively binds to the axoneme in an end-to-end manner. These and other results indicate that cooperative association of the ODA-DC underlies the periodic OAD arrangement at specific positions on the doublets. These findings provide insight into how the regular axonemal repeat structure is produced.</jats:p

Crossref

Proceedings of the National Academy of Sciences

Cooperative binding of the outer arm-docking complex underlies the regular arrangement of outer arm dynein in the axoneme

http://www.pnas.org/content/111/26/9461.full.pdf

Cooperative binding of the outer arm-docking complex underlies the regular arrangement of outer arm dynein in the axoneme

Abstract

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