Anderssonson, Magnus

Crowe, Mark

Ghanem, Nasser

Hamama, Tuula-Marjatta

Honkatukia, Mervi

Kantanen, Juha

Li, Meng-Hua

Peippo, Jaana

Pokharel, Kisun

Rautiainen, Johanna

Seppälä, Arja

Jukuri

P0421: Ovarian mRNA and miRNA Transcriptome Profiling of Domestic SheepBreedsThe highly prolific breeds of domestic sheep (Ovis aries) are valuable genetic resources for global sheep industry. E.g.the native Finnsheep is well-known for its prolific traits and has been imported into 40 countries to develop new breedsand to improve fertility of local sheep breeds. Female prolificacy traits of sheep, such as the high ovulation rate andlitter size, are critical factors which effect on biological and financial performance of sheep production. To improve ourknowledge of the sheep prolificacy traits, we analysed mRNA and miRNA sequences of ovarian tissues from two purebreeds with large (Finnsheep) vs. small (Texel) litter sizes and their F1 crosses. Half of the ewes in each group werefed a flushing diet in order to investigate the effect of feeding on ovulation rate. We found that among the samples,16,402 genes (60.6% known ovine genes) were expressed. We detected 79 novel miRNAs and a cluster of miRNAson chromosome 18. The majority of the differentially expressed genes between breeds were upregulated in the low-prolific Texel, owing to the flushing diet effect, whereas a similar pattern was not detected in the Finnsheep. F1 ewesresponded similarly to Finnsheep rather than exhibiting a performance intermediate between the two pure breeds. Weobserved in detail the variants of four major candidate genes for prolificacy (GDF9, BMP15, BMPR1B and B4GALNT2)identified previously in sheep. None of the previously studied mutations at these candidate genes were present in anyof the samples. Interestingly, the mutation V371M in GDF9 (C-T transition at 5:41841285) was present in fiveFinnsheep and four F1 crosses but was not present in Texel. In addition, the three genes (CST6, MEPE and HBB) thatwere differentially expressed between the group of Finnsheep and Texel ewes kept in normal diet appeared to becandidate genes of prolificacy traits and will require further validation.AuthorsKisun PokharelJaana PeippoMervi HonkatukiaArja SeppäläJohanna RautiainenNasser GhanemTuula-Marjatta HamamaMark CroweMagnus AnderssonMeng-Hua LiJuha KantanenNatural Resources InstituteFinlandNatural Resources InstituteFinlandNatural Resources InstituteFinlandEastman Chemical CompanyLammasmaailma OyNatural Resources InstituteFinlandCairo UniversityNatural Resources InstituteFinlandUniversity College DublinUniversity of HelsinkiChinese Academy of SciencesNatural Resources InstituteFinlandView Related Events

Ovarian mRNA and miRNA Transcriptome Profiling of Domestic Sheep Breeds

http://jukuri.luke.fi/bitstream/10024/543523/1/Plant%20and%20Animal%20Genome%20XXVI%20Conference%20%28January%2013%20-%2017%2c%202018%291.pdf

Ovarian mRNA and miRNA Transcriptome Profiling of Domestic Sheep Breeds

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