Bacteriophage are a diverse class of viruses that infect bacterial cells. As a result of over 60
years of molecular biology advances, bacteriophage today feature as candidates for vaccination,
gene therapy, biomaterial and antibacterial purposes. Consequently, scientific, commercial and
public awareness of bacteriophage is growing rapidly. There is now an increasing need for the
establishment of strong biochemical engineering foundations to serve as a guide for future
bacteriophage bioprocessing. It has been the purpose of this study to contribute towards this
knowledge base, by understanding the properties of the filamentous bacteriophage M13.
Ultimately, this work has aimed to allow for the more efficient assembly of a large-scale
production process.
By the application of well-understood small-scale predictive techniques, it has been found that
bacteriophage M13 should not be severely damaged by hydrodynamic shear forces of the
duration and magnitude imparted by fermentation, pumping or continuous centrifugation
operations. Thus, it may well be possible to manufacture on the large-scale using existing large-scale
equipment designs.
Amongst bacteriophage, the reproduction strategy of M13 is unusual in that propagation occurs
by the non-lethal extrusion of progeny through the cell wall of the E. coli host. Investigation of
bacteriophage M13 propagation indicated that growth in a medium that increased host cell
density concomitantly increased bacteriophage yield; a four-fold increase to 2 x 1012 pfu ml-1
was achieved. At the end of culture, concentrations of supernatant DNA and protein
contaminants were found to vary amongst three E. coli strains studied.
Post-fermentation, bacteriophage M13 can be precipitated from the cell-free process fluid by as
little as 2 % (w/v) PEG 6 000 plus 25 mM magnesium sulphate, or by isoelectric precipitation.
Purification factors in excess of 100 were achieved by PEG-salt precipitations with regards to
the reductions in DNA and protein concentrations.
Methods used in this study have increased the processing knowledge of bacteriophage M13 and
have a broader applicability to both derivatives of M13 and other bacteriophage