The slow rusting wheat cultivars viz., Tukuru and Pavon-76 were hybridized with a widely adopted Pakistani wheat cultivar ?Inqilab-91? to incorporate durable rust resistance. Specific primers were used to validate the presence of durable rust resistance in a bulked segregating population. The F1 plants were backcrossed with either parent to obtain BC1F1 populations which were then self-pollinated to get four F2 populations. A total of 102 randomly selected back-crossed BC1F2 plants were used for bulk segregant?s analysis. Genomic DNA was extracted from randomly selected individual plants and aliquots of DNA of each sample were mixed to constitute bulk pools of each population. Durable resistance gene Lr34/Yr18 was heterozygous in populations 1, showing amplification of both the diagnostic csLV34a allele and the null csLV34b alleles. Population 2 amplified homozygous csLV34a alleles, while populations 3 and 4 amplified the null allele. The Lr46/Yr29 gene was identified in population 3 and 4. Compared to Inqilab-91 the Population 1 indicated 33-67% and Population 2 indicated 56-78% lower infection type while population 3 and 4 indicated 22-44% lower infection type to yellow rust. All the populations and parents showed the prevalence of Sr2 and absence of Lr37/Yr17/Sr38 gene complex. ? 2017 Friends Science PublisherspreprintPeer reviewe
