Nisikado, Yosikazu

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A Simple Device for Drawing and Photomicrographing Small Living Spores of Fungi.1)

Okayama University Scientific Achievement Repository

Band V. Heft生.Berichte des Ohara Instituts fur landwirtschaftliche Forschungen 1933 A Simple Device for Drawing and Photomicrographing Small Living. Spores of Fungi.1 By Yosikazu Nisikado.(72) [Jnn1lRry 27， 1933.] Pho色omicrographingfungu8-spores and any other圃mallbodie圃 moun旬din w叫er，or drawing them with an aid of a camera lucida is nece自抽，ryin many c制 esfor those who are engaged in the plant pathology and microbiology. Small fungu自・自pore自or圃uch圃mallbodie自由howBrownian movemenもb自由ide自由emove-menもdue色othe gravityand出ewater current， when出eyare mOUI陶 din water， 80出叫 iもi自 verydifficulももotake出。irpho旬micrograph. To overcome出edi盤cu出;y，a device had been tried by自ealingthe margin of cover gl朗自 wi出vaseline or p町 a血n.In 1927 SBERB岨 O町 (1927)proposed a new me出odfor pho加graphingthe preparations of living自pore自国inga出inlayer of plain agar. The SBERB岨 OFF'Smethod wa自由ogood旬 getrids of the gr岨も par旬 oftheもroublesin pho加micro-graphing the living圃pore圃， and the present writer (NmIl!:ADO， 1927) has introduced thi自 method with his modif.cation自・ The method w制 ciもedby TAKIMOTO (19却}in his“Me也od自inMicrobiology and PlanもPathology".1) This i自由 Englishtmnslation of the writer's {:凪戸rwritten in J"p8nese. in "No伊ku-Kenky色.. (Agrfo叫tumlRes岨roh)，Vol. 19， pp. 3o9-360 pubushed from the Oham Institute， Bept. 1. 1932. 480 Y. NIBIlUOO: Although SHERBAKOFF'S meもhodgive日 aprett.y good result for the phoω-micrographing or drawing日mallliving spores，出epractice i自白omewhattedious. N阻 elyby thi日method，theもube自ofcarefully filもeredcl叫 ragar mu日tbe always available， since出em自由odrequir倒 athin layer of agar every t泊e. An old agar layer may become dry or contaminated， although出eagar layer on the slid白 mayb自goodfor some days u they are pre随rvedwell. Moreover it is a.lso troubl白日ome旬 get出eagar layer complet白ly自ven.To eliminate出伺etroubles， the present writer has仕ieda collodion film in pl郎自 of出ethin layer of agar， and obtain白dpretty good resul匂. This new method i日 verysimple and convenient for photomicrographing or for camera-lucida-drawing of smaU living sporωmounted in water. The manipulation of thIBm自由odis as follows : 1) D江utecollodion田 lution(commercial)加 4加 5times with th白 mixtureof lp町 tof absolute alcohol and 2 par旬 of自由自，r.2) Dip a cleanly wiped and dried cover glass in the above collodion solution. wi出 anaid of forceps， and then drain 0貸出自由urplusof日olutionas comple旬 asp個目ble.3) Put the ∞ver glass on blot出 gpaper， evapora旬 etherand alcohol 60 th叫the thin collodium film adher倒 tothe cover gla関.4) Remove the collodion film on on自由ideof the cover gla飽 witha prepara-tion needle or a knue. 5) Put a small drop of water on the center of collodion layer on the ∞ver glass th田 prepared. Mix the spores to be observed in the drop加 makethe spore suspension; keep the susp自国ionnearly dry・6) Transfer the cover gl朗自 ona drop of watぽ ona slide gl朗自， of course， the film side of the cover gl脇島 vis-a-vIBthe slide; preωit properly. Blot off the surplu自ofwater. ηSeal the margin of the cover gla.闘wi出 melもedparaffin. The preparation. is now ready for the pho旬micrographingor for the camera・lucida-drawing.Referencea. . SBEBBAXOFJ'， O. D. 1927 Photomlorographing living oonidia monnted on agar. Phytopnthol句0'， 17:3t 195-198. NISI也 00，Y. 1927 On photomiorogmphillg of spores of pnthogenetio fnnl訴 Journalof Plant P川崎ti四 ，14:317-320. 1，，/0ψ加 M・1932 A simple devioe for photomior<宅raphingBnd dmwing living funguB-spores. Agrioultural B倒曲roh，19: 359-360. 1" Japa"~u， TAXrMO'l'O，S. 1930 Methods in Mforobiology Bnd Plant P..thology. 390 pp. (Photomiorographing of spores， pp. 43-44). 1" Jaj倒的.

A Simple Device for Drawing and Photomicrographing Small Living Spores of Fungi.1)

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