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Quantitative studies of nucleic acid in the cell by micro-spectrophotometry I. The critique and improvement of the microspectrophotometry

Abstract

Some critical experiments have been carried out on the microspectrophotometry using the lymphocytes of a mouse, stained with Feulgen reaction, revealing that most reliable value can be attained by illuminating the material with a small spot-light and integrating the area surrounded by the extinction curve drawn by tracing along the diameter of the smeared and fixed cell.</p

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