Since Hahn's observation of the postalimentary lipemia clearing actIvity following the injection of heparin, physiological, biochemical and clinical significances
of the postheparin lipoprotein lipase have been well clarified. The presence of the endogenous lipoprotein lipase in human blood, which was at first doubted, has been repeatedly confirmed2&#8764;8. Recent papers9,10 described elevated endogenous lipoprotein lipase activity in patients with essential hyperlipemia after ample fat uptake. In this preliminary report, changes of the lipoprotein lipase activity during oral glucose tolerance test is illustrated.</p

Kawanishi, Koichi

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Changes of the endogenous lipoprotein lipase activity during oral glucose tolerance test

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Acta Medica OkayamaVolume 21, Issue 4 1967 Article 5AUGUST 1967Changes of the endogenous lipoprotein lipaseactivity during oral glucose tolerance testKoichi Kawanishi∗∗Okayama University,Copyright c©1999 OKAYAMA UNIVERSITY MEDICAL SCHOOL. All rights reserved.Changes of the endogenous lipoprotein lipaseactivity during oral glucose tolerance test∗Koichi KawanishiAbstractSince Hahn’s observation of the postalimentary lipemia clearing actIvity following the injec-tion of heparin, physiological, biochemical and clinical significances of the postheparin lipoproteinlipase have been well clarified. The presence of the endogenous lipoprotein lipase in human blood,which was at first doubted, has been repeatedly confirmed2∼8. Recent papers9,10 described el-evated endogenous lipoprotein lipase activity in patients with essential hyperlipemia after amplefat uptake. In this preliminary report, changes of the lipoprotein lipase activity during oral glucosetolerance test is illustrated.∗PMID: 4230850 [PubMed - indexed for MEDLINE] Copyright c©OKAYAMA UNIVERSITYMEDICAL SCHOOLActa Moo. Okayama 21, 185-189 (1967)BRIEF NOTESCHANGES OF THE ENDOGENOUS LIPOPROTEIN LIPASEACTIVITY DURING ORAL GLUCOSE TOLERANCE TESTKoichi KAWANI SRIDepartment of Internal Medicine, Okayama University Medical School,Okayama, Japan (Director: Prof. K. Hiraki)Received for publication, July 26, 1967Since Hahn's observation of the postalimentary lipemia clearing actIvityfollowing the injection of heparin, physiological, biochemical and clinical signi-ficances of the postheparin lipoprotein lipase have been well clarified. Thepresence of the endogenous lipoprotein lipase in human blood, which was atfirst doubted, has been repeatedly confirmed2- s• Recent papers9. 10 describedelevated endogenous lipoprotein lipase activity in patients with essential hyper-lipemia after ample fat uptake.In this preliminary report, changes of the lipoprotein lipase activity duringoral glucose tolerance test is illustrated.Measurement of the lipoprotein lipase (LPL) activity was performed bydetermination of the release of free fatty acid (FFA) in vitroll - 13•In normal persons (10 cases) (Fig. 1), no LPL activity was demonstratedin the plasma before and 30, 120 and 180 minutes after glucose intake. And,elevated LPL activity was observed at 60 and 90, and 150 minutes after glucoseintake, showing two peaks of the LPL activity. After incubation (at 37°C forhalf an hour) of the plasma mixed with sesame oil (Fatgen), the identical changesof the LPL activity curve were observed. But the peaks of the enzymatic acti-vity of the postincubation plasma were about three times as high as that of theprcin~ ubation plasma.In diabetics (9 cases) (Fig. 2), the LPL activity in plasma before incubationwas quite different from changes in normal persons. No activity was observedbefore glucose intake, but the activity in the plasma rose at 30, 60 and 90minutes, when the activity reached a peak, and then the activity decreased 120,150 and 180 minutes after glucose intake, finally showing almost no activity.The elevation of the enzymatic activity in diabetics was higher than that in nor-mal persons. After incubation of the plasma mixed with sesame oil (Fatgen),identical changes of the LPL activity curve were also observed, demonstrating1851Kawanishi: Changes of the endogenous lipoprotein lipase activity during oralProduced by The Berkeley Electronic Press, 1967186pEq/ I400300200100K. KAWANISHI••·•..D I.><....././.mg/dl15010050o 30 60 90 120 150 180 minutesFig. 1 Plasma LPL and FFA and blood sugar levels in normal personsA: Preincubation LPL activity (flEq/L) B: Postincubation LPL activity (flEq/L)C: (Postincubation LPL activity)-(Preincubation LPL activity) (flEq/L) D: FFA(flEq/L), E: Blood sugar (mg/dl)pEq/L600500400300200100E./.~."" ..-. ......../ ......../ B "',.? ....?:..... '.f '.;' .•...f,.mg/cll300200100o 30 60 90 120 150 180 minutesFig. 2 Plasma LPL and FFA and blood sugar levels in diabetics.A: Preincubation LPL activity (flEq/L) B: Postincubation LPL activity (flEq/L),C: (Postincubation LPL activity)-(Preincubation LPL activity) (flEq/L) D: FFA(JLEq/L), E: Blood sugar (mg/dl)2Acta Medica Okayama, Vol. 21 [1967], Iss. 4, Art. 5http://escholarship.lib.okayama-u.ac.jp/amo/vol21/iss4/5LiIX>protein Lipase Activity 187}lEq /L mg/dl800.A.. 200/ i '.J: '.700 \600 \ 150\\500 \..\,400 V· 100:,,--300200 50100o 30 60 90 120 150 180minutesFig. 3 Plasma LPL and FFA and blood sugar levels in patients with cirrhosis of liverhaving diabetic GTT curve.A: Preincubation LPL activity (J.lEqjL) B: Postincubation LPL activity (J.lEq/L),C: (Postincubation LPL activity)-(Preincubation LPL activity) (pEqjL) D: FFACJ.lEqjL), E: Blood_sugar (mgjdl)}lEqjL700 B600500400300200100mg/dl15010050o 15 30 45 60 90 120 minutesFig. 4 Plasma LPL and FFA and blood sugar levels in diabetic during insulin sensitivitytest.A: Preincubation LPL activity CJ.lEq/L), B: Postincubation LPL activity CJ.lEq/L),C: (postincubation LPL activity) - (Preincubation LPL activity) CJ.lEq/L) D. FFA(pEq/L). E: Blood sugar (mgjdl)3Kawanishi: Changes of the endogenous lipoprotein lipase activity during oralProduced by The Berkeley Electronic Press, 1967188 K. KAWANISHIa single peak at 90 minutes and no activity before and after glucose tolerancetest. The LPL activity of the postincubation plasma was elevated about twiceas high as that of the preincubation plasma. But differences of the LPL activitybetween the pre- and postincubation plasma showed almost same value in normalpersons and diabetics.FFA levels were decreased until 150 minutes and elevated at 180 minutesduring oral GTT in normals. In diabetics, FFA levels remained elevated for30 minutes and then .decreased until 150 minutes and again elevated at 180minutes, keeping higher levels than normals throughout oral GTT.In patients (3 cases) with cirrhosis of the liver having diabetic GTT curve(Fig. 3), the LPL activity of the preincubation plasma showed a single peak curveas in diabetics, but the LPL activity of the postincubation plasma demonstratedconsiderably elevated activity before GTT and 180 minutes after glucose intake.At these moments no LPL activity was usually revealed both of the normal anddiabetic. The enzymatic activity curve showed a single peak curve having thehighest level at 90 minutes as in diabetics.In insulin sensitive test (Fraser's method14) in the diabetic patient (Fig. 4),an identical LPL activity curve as in oral GTT of diabetics was observed in spiteof lowered blood sugar level.These enzymatic activities were completely inhibited by the administrationof protamine sulfate and 10 per cent NaCI solution in vitro.These observations are clearly demonstrated that this LPL is the endogenouslipoprotein lipase activated by glucose metabolism and strongly suggested thatthis enzyme has some sorts of close relationship with insulin activity in thehuman subject.The endogenous LPL activity was also observed during fat tolerance testand food uptake.ACKNOWLEDGEMENTGrateful acknowledgement is made to Prof. K. HIRAKI for his constant interest and guidancein this investigation.REFERENCES1. HAHN, P. F.: Abolishment of alimentary lipemia follwing injection of heparin. Science 98,19, 19432. ENGELBERG, H. : Human endogenous lipemia clearing activity. Study of lipolysis and effectsof inhibitors. ]. BioI. Chem. 222, 601, 19563. CLELAND, W. W. and IACONO, J. M. : Role of lipoprotein lipase in intravascular clearing offat in human subjects. Fed. Proc. 16, 383, 19574. GATES, H. S., JR. and GORDON, R. S., JR.: Demonstration of lipoprotein lipase in fastinghuman serum. Fed. Proc. 17, 437, 19584Acta Medica Okayama, Vol. 21 [1967], Iss. 4, Art. 5http://escholarship.lib.okayama-u.ac.jp/amo/vol21/iss4/5Lipoprotein Lipase Activity 1895. GUNNING, B., et al.: Lipoprotein lipase in norm- and hyperlipidemic humans (abstr., No.2).Fed. Proc. 21, 285, 19626. HOOD, B. et al. : Lipoprotein and spontaneous lipoprotein lipase activity on inhibition inhuman plasma. ]. Atheroscler. Res. 2, 438, 19627. CSEH, G. and SZABO, I. K.: Appearance of lipoprotein lipase activity in human nonposthe·parin serum. Clin. Chim. Acta. 8, 382, 19638. ENGELBERG, H.: Heparin and the removal of triglyceride from the blood stream. Am. ].Cardiol. 14, 8, 19649. SANDHOFER, V. F. et al.: Endogene LipoproteidlipaseaktiviUit in menschlichem Plasma.Dtsch. Med. Wschr. 89, 426, 196410. SAILER, V. S. et al.: Steuerung der endogenen Lipoproteid-Lipase-Aktivitat im Plasma beiNormalpersonen und Patienten mit essentieller Hyperlipamie. Dtsch. Med. Wschr. 90,865, 196511. DoLE, V. P.: A relation between non-esterified fatty acids in plasma and the metabolismof glucose. f. Clin. Invest. 35, 150, 195612. DoLE, V. P, and MEINERTZ, H.: Microdetermination of long·fatty acids in plasma andtissues. ]. Bioi. Chem. 235, 2595, 196013. OKANIWA, H. : Lipoprotein lipase. Rinsho Kosogaku, p.483, Tokyo, 1964 (in Japanese)14. FRASER et al.: ]. Clin. Endocr. 1: 297, 1Q41, Cited by Kanai, 1. Rinsho KensahoTeiyo. p. XV-30, Tokyo, 1966 (in Japanese)5Kawanishi: Changes of the endogenous lipoprotein lipase activity during oralProduced by The Berkeley Electronic Press, 1967

Changes of the endogenous lipoprotein lipase activity during oral glucose tolerance test

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