Kawanishi, Koichi

Ofuji, Tadashi

Okada, Soji

Okayama University Scientific Achievement Repository

Acta Medica OkayamaVolume 28, Issue 5 1974 Article 8OCTOBER 1974Formation of antibody to monocomponentinsulin in guinea pigsSoji Okada∗ Koichi Kawanishi†Tadashi Ofuji‡∗Okayama University,†Okayama University,‡Okayama University,Copyright c©1999 OKAYAMA UNIVERSITY MEDICAL SCHOOL. All rights reserved.Acta Merl. Okayama 28, 377-378 (1974)--BRIEF NOTE--FORMATION OF ANTIBODY TO MONOCOMPONENTINSULIN IN GUINEA PIGSSoji OKADA, Koichi KAWANISHI and Tadashi OFUJIDepartment oj Internal Medicine, Okayama University Medical School,Okayama, Japan (Director: Prof. T. Ojuji)ReceivedJor publication, July 22, 1974It has been well known that conventional insulin preparations induceinsulin antibodies in man and animals. Recently SCHLICHTKRULL et al. (1)demonstrated lack of antigenicity of monocomponent insulin (MC insulin) inrabbits after immunization with soluble MC insulin alone. The followingstudy was designed to clarify the antigenicity of the MC insulin in guinea pigs.Fifteen male guinea pigs were divided into three groups of five animalseach. Group I received injections of 0.5 ml of Freund's complete adjuvantevery two weeks for four weeks in six subcutaneous areas. Group II wasinjected with 20. 0 IU of the MC insulin mixed with O. 5 ml of Freund'scomplete adjuvant. And Group III was given conventional pork insulin withthe adjuvant in the same way. Serum samples were collected by cardiacpuncture. Insulin antibody was assayed by using 125I-insulin and ethanolprecipitation (2) in an 1 : 26 dilution of the serum. The antibody titer wasindicated by the percentage of bound/total count.Four of five guinea pigs immunized with the MC insulin induced anti-body formation (Fig. 1). Following the first injection of the MC insulin,binding antibody to the insulin appeared already to some degree in two offive animals. Upon the second exposure to the MC insulin, the level of theantibody titers rose rapidly and it was 25. 0% in guinea pigs. While that tothe conventional insulin was 31.2% and Group I showed no elevation of anti-body titers.Insulin easily produces -antibody and it has been also speculated thatproteins of high molecules or polypeptides contained in the conventional insulinpotentiate the formation of antibody to insulin. The present study showedthat the formation of binding antibody to insulin was observed in the MC aswell as in the conventional insulin, both of which had been given togetherwith Freund's complete adjuvant. From this finding, it appears that theabsence of contaminants in the MC insulin is not sufficient to make the insulinnon-immunogenic. The observation of HIRATA (3) that three of the five rabbits3771Okada et al.: Formation of antibody to monocomponent insulin in guinea pigsProduced by The Berkeley Electronic Press, 1974378 S. OKADA, K. KAWANISHI and T. OFUJICONVENTIONALMC INSULIN INSULINz GO GOH...l 50 50:::>rflzH 40 40IHl/"')N 30 300z 20 20:::>0c:lLl.. 1 0 1 00b~0 2 3 4 0 2 3 4~ ~ ~ ~WEEKS OF IMMUNIZATIONFig. 1. Formation of binding antibody to MC insulin in guinea pigs.immunized with MC insulin together with Freund's complete adjuvant deve-loped insulin antibody also coincides with our results.Insulin preparations in a modified form or in acid solution readily induceantibody. And it is well known that the use of an adjuvant potentiates anti-body formation (4). However, insulin preparations in the soluble form or inthe neutral solution result in weak antigenicity (5). SCHLICHTKRULL et ai. (1)immunized rabbits with the MC insulin dissolved in neutral solution- and didnot use the adjuvant, so that under their conditions it may be that they couldnot detect any antibody binding to insulin.It seems reasonable to conclude from the present study that antigenicityof insulin is not induced by the contaminants in the insulin preparations, butby the insulin itself.Acknowledgement: Thanks are tendered to Prof. Y. HIRATA of Tottori University forproviding the MC pork insulin.REFERENCES1. SCHLICHTKRULL, J., BRANGE, J., EGE, H., HALLUND, 0., HEDlNG, L.G., J¢RGENSEN, K.H.,MARKUSSEN, J., STAHNKE, P., SUNDBY, F. and Y'/JLUND, Aa.: Proinsulin and related pro-teins. Lecture held at the 5th Annual Meeting of European Association for the Studyof Diabetes, Montpellier, 16th-18th September, 19692. WELBORN, T. A., RICHARDS, R. and FRASER, T. R.: Simple test for insulin antibodies insera, using 131I-insulin and ethanol precipitation. Brit. Med. J. 1, 719, 19673. TOMINAGA, M. and HIRATA, Y.: Insulin antibody formation and glucose tolerance inthe rabbits immunized with a- or b- component in crystalline bovine insulin. J. JapanDiab. Soc. Supplement. 17, 227, 1974 (In Japanese)4. MAKULU, D. R. and WRIGHT, P.: Immune response to insulin in guinea pigs. Metabolism20, 770, 19715. DECKERT, T. and GRUNDAHL, E.: The antigenicity of pig insulin. Diabetologia 6, 15, 19702Acta Medica Okayama, Vol. 28 [1974], Iss. 5, Art. 8http://escholarship.lib.okayama-u.ac.jp/amo/vol28/iss5/8

Formation of antibody to monocomponent insulin in guinea pigs

Abstract

Similar works

Full text

Available Versions

Okayama University Scientific Achievement Repository

Okayama University Digital Information Repository