Kamimura, Okinobu

Kitajima, Koichi

Mikochi, Hiroshi

Nakanishi, Toshio

Sakato, Junya

Takahashi, Isao

Toki, Hironobu

Okayama University Scientific Achievement Repository

Acta Medica OkayamaVolume 29, Issue 4 1975 Article 8AUGUST 1975Effect of neocarzinostatin on hemolyticplaque-forming cell production in miceIsao Takahashi∗ Hiroshi Mikochi† Junya Sakato‡Toshio Nakanishi∗∗ Hironobu Toki††Okinobu Kamimura‡‡ Koichi Kitajima§∗Okayama University,†Okayama University,‡Okayama University,∗∗Okayama University,††Okayama University,‡‡Okayama University,§Okayama University,Copyright c©1999 OKAYAMA UNIVERSITY MEDICAL SCHOOL. All rights reserved.Acta Merl. Okayama 29, 327-328 (1975)-- BRIEF NOTEEFFECT OF NEOCARZINOSTATIN ON HEMOLYTICPLAQUE-FORMING CELL PRODUCTION IN MICEIsao TAKAHASHI, Hiroshi MIKOCHI, Junya SAKATO, Toshio NAKANISHI,Hironobu TOKI, Okinobu KAMIMURA and Koichi KITAJIMASecond Department of Internal Medicine, Okayama University Medical School,Okayama, Japan (Director: Prof. Kiyoshi Hiraki)Receivedfor publication, April 18, 1975Neocarzinostatin (NOS), a new anti-leukemic substance with a highmolecular weight, was first isolated from culture filtrates of Streptomycescarzinostaticus (1). Its anti-leukemic activity has been demonstrated experi-mentally in various mice leukemias (2,3). Its effect, however, on the immuneresponse has not been reported. Our preliminary data reported here on miceindicates that NOS has a definitive effect on the production of hemolyticplaque-forming cell (HPFO).Mice used were 1.5-2.0 month-old 057BL males, weighing 20-22 g each.The mice were sensitized by i. p. injection of 0.2 ml of 20% suspension ofsheep red blood cells (SRBC) on Day O. NOS at 1.0 mg/kg was injected i. p.once to six different experimental groups: Group A-12 days prior to SRBOinjection; Group B-8 days prior to SRBO injection; Group 0-4 days priorto SRBO injection; Group D-on the day of SRBC injection: Group E-2days after SRBC injection and Group F-4 days after SRBC injection. Con-trol mice were injected with 0.2 ml physiological saline solution. On day 5,HPFC assay was perfonned according to the Cunningham and Szenberg (4)plaque technique. As shown in Table 1, the mean HPFC per 106 spleencells was 348.6 in Group A, 8.9 in Group B, 8.8 in Group C, 265.9 in GroupD, 358.8 in Group E, 465.6 in Group F and 993.5 in the control group.These results clearly show a moderate to marked decrease of HPFCproduction regardless of the time duration between SRBC and NCS admini-stration. NCS seems to have a suppressive effect as some alkylating agents.The main anti-leukemic action of NCS has been thought to be through inhibi-tion of DNA synthesis (5). However, our results suggest that NOS has a directcytotoxic action on leukemic cells apart from the inhibition of DNA synthesis.However, further study including NOS effects on cellular immunity areneeded prior to definitive conclusions.3271Takahashi et al.: Effect of neocarzinostatin on hemolytic plaque-forming cellProduced by The Berkeley Electronic Press, 1975328 1. TAKAHASHI et al.TABLE 1 EFFECT OF NEOCARZINOSTATIN (NCSj ON HEMOLYTIC PLAQUE-FORMINGCELL (HPFC) PRODUCTION OF MICE TO SHEEP RED BLOOD CELLNCS administration Number Total spleen HPFC per 106 HPFC peron day of mice cell (x 106) spleen cells spleen (x 103)-12 3 Ill. 7 348.6 49.9(63.8-151. 3) (59.5-879.7) ( 3.8-133. I)8 2 55.5 8.9 0.8(21. 0-90.0) ( 0-17.8) ( 0-1.6)4 3 56.7 8.8 0.6(25.0-75.0) ( 0-18.5) ( 0-1. 3)0 3 61. 3 265.9 20.4(38.8-82.5) (64.4-608.6) ( 2.5-51.0)+ 2 3 68.8 358.8 28.0(23.8-98.8) (6.1-965.4) ( 0.6-80.9)+ 4 3 66.2 465.6 35.0(48.5-80.0) (62.9-1140.0) ( 4.4-91.2)Control(treated by saline) 3 84.6 993.5 85.6(78.8-93.8) (630.7-1293.2) (49.7-121. 3)-------REFERENCES1. Ishida, N., Kumagai, K., Miyazaki, K. and Ito, M.: Carzinostatin, a new anti-tumorsubstance, Cann 51 (Supple) 56, 1960.2. Takahashi,1., Sakato, J., Mikochi, H., Moriwaki, H., Kitajima, K., Irino, S. and Hiraki,K.: Antitumor activity of Neocarzinostatin; Effect on Rauscher leukemia in mice. ActaMed. Okayama 2", 271, 1974.3. Bradner, W. T. and D. J. Huchinson: Neocarzinostatin (NCS-69856l: Antitumor anti-biotic effective against ascitic leukemia L 1210 in mice. Cancer Chernoth. Rep. , , 79, 19664. Cunningham, A. J. and Szenberg, A.: Further improvement in the plaque technique fordetecting single antibody formation cells. Immunology H~, 599, 1968.5. Ono, Y., Watanabe, T. and Ishida, N.: Mode of action of Neocarzinostatin; inhibitionof DNA synthesis and degradation of DNA in Sarcina lutea. BioP,!'Ys. Biochem. Acta 119,46, 1966.2Acta Medica Okayama, Vol. 29 [1975], Iss. 4, Art. 8http://escholarship.lib.okayama-u.ac.jp/amo/vol29/iss4/8

Effect of neocarzinostatin on hemolytic plaque-forming cell production in mice

Abstract

Similar works

Full text

Available Versions

Okayama University Scientific Achievement Repository