Cellulase is a reduced enzyme synthesized from microorganisms grown in cellulose medium. Cellulase and β-glucosidase enzymes are present in various types of bacteria, fungi and yeasts. Strains of yeast Debaryomyces Hansenii and Debaryomyces Polymorphus show high β-glucosidase activity while Aspergillus Niger fungi produce high cellulase activity and less-glucosidase. Cellulase enzyme can hydrolyze cellulose into glucose by fermentation. In this study, enzyme mixing of yeast fermentation and fermentation of fungi was done. Strain of Debaryomyces Hansenii has the highest β-glucosidase content in 4 days production of enzyme 0,2650212 units / ml mixed with Aspergillus Niger L-76 0,0592 units / ml fungi for kinetics test of mixed enzyme reaction with yeast ratio: fungi = 1: 1, 1: 2 and 2: 1. The highest mixed enzymes were produced at a 2: 1 ratio and had the enzyme kinetic parameters of Vmax of 0.200080032 g / L and Km 1.589636L / g using a variation of CMC substrate concentration of 0.3; 0.4; 0.5; 0.6; 0.7; 0.8; 0.9; and 1g / L
