This article aims to discuss the main roles of natural history museums and to show how these purposes have evolved and adapted throughout the museums’ history, as a response to the development of natural sciences and societal change, from their creation in the 18th century to the present. It strives to demonstrate how the balance between research, teaching and disseminating knowledge to the public has successively shifted, without ever forsaking any of these functions. It is focused on Portuguese museums, but examining their place within international trends