Osteogenesis process displays a fundamental role during dental implant osteointegration.
In the present work, we studied the influence of Osteon Growth Induction (OGI) surface
properties on the angiogenic and osteogenic behaviors of Mesenchymal Stem cells (MSC).
MSC derived from dental pulp and HUVEC (Human Umbilical Vein Endothelial Cells) were
grown in on OGI titanium surfaces, and cell proliferation and DNA synthesis were evaluated by
MTT [3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide] test and DNA quantification.
Gene expression has been performed in order to evaluate the presence of mRNA related to endothelial
and osteogenesis markers. Moreover, morphological and biochemical analyses of osteogenesis
commitments has been performed. On OGI surfaces, MSC and HUVEC are able to proliferate.
Gene expression profiler confirms that MSC on OGI surfaces are able to express endothelial and
osteogenic markers, and that these expression are higher compared the expression on control
surfaces. In conclusion On OGI surfaces proliferation, expression and morphological analyses
of angiogenesis-associated markers in MSC are promoted. This process induces an increasing on
their osteogenesis commitmen