The aim of this paper was to test the effect of three plant growth regulators (PGR) on fiber content of hemp cultivar Helena. Applied PGRs, Cycocel® , Regalis® and Moddus® had impact on fiber content in hemp cultivar Helena in medium concentrations: 1-2, 1.25-2.5 and 0.4-0.5 kg/ha, respectively. Further analysis of fiber quality, after application of various PGRs concentrations, should explain which PGR should be used in hemp fiber production