Several photosynthetic pigments from two algae, namely Padina pavonica and Codium fragile, were extracted in different solvents and subsequently analyzed through TLC. The optimization of the separation parameters led to the choosing of the optimum eluent (hexane/acetone mixture) for polar silica gel plates. The isolated compounds were evaluated through densitometric measurements and by the acquisition of their UV-Vis spectra. While xanthophylls, chlorophyll a and pheophytin a were the most typical pigments of Padina pavonica, chlorophyll a and b, xanthophyll and β-carotene were the most characteristic pigments for Codium fragile