Efficient leaching of cellulases produced by Trichoderma harzianum in solid state fermentation

Abstract

Recovery of cellulases from solid state culture of #Trichoderma harzianum$ was efficiently achieved by hydraulic pressing. Pressing of fermented solids yielded carboxymethyl-cellulase (CMCase) extraction efficiency of 71% and a ratio of leachate to fermented solids of 0.58 (v/w). Addition of water to pressed solids and second pressing improved the efficiency (95%) with simultaneous increase in the ratio to 1.16 (v/w). The overall extraction of filter paper activity was lower (85%) than that of CMCase. This technique is simple and its extraction efficiency is similar to that obtained in multiple-contact counter-current systems. The hydraulic press in its individuality was not used earlier to leach the product from fermented solids. (Résumé d'auteur