Effect of olive oil on antioxidative defense components in liver of cadmium intoxicated rats

Abstract

The present study investigated the possible protective role of olive oil on antioxidative defense components in liver of cadmium (Cd) intoxicated animals. Male, Wistar albino 3 months old rats were injected with a single dose of: 1) CdCl2 (0.4 mg Cd/kg i.p. and sacrificed after 24h), 2) olive oil (0.4 ml olive oil/kg b.m., i.m., 48h before the sacrificing) and 3) olive oil + Cd (in above mentioned amounts). Treatment with Cd increased lipid peroxidation (LP) in blood and activities of alanine and aspartate transaminase (ALT and AST) in plasma. Concentrations of non enzymatic components of antioxidative defense system (AOS): vitamin C (Vit C) and vitamin E (Vit E) were significantly decreased in liver tissue of Cd treated rats. Pretreatment with olive oil reversed Cd induced alterations in LP content and transaminases activities, as well as on endogenous antioxidive defense components.U ovom radu ispitivana je moguća zaštitna uloga maslinovog ulja na antioksidacione zaštitne komponente u jetri pacova intoksiciranim kadmijumom (Cd). Mužjaci pacova Wistar albino, starosti tri meseca akutno su tretirani sa: 1) CdCl (0.4 mg Cd/kg t.m., i.p., 24h pre žrtvovanja), 2) maslinovim uljem (0.4 ml maslinovog ulja/kg t.m., i.m., 48h pre žrtvovanja) i 3) maslinovim uljem + Cd (u navedenim količinama). Posle tretmana kadmijumom značajno se povećava koncentracija lipidnih peroksida (LP) u krvi, kao i aktivnost alanin i aspartat transaminaza (ALT i AST) u plazmi. Koncentracije neenzimskih komponenti antioksidacionog zaštitnog sistema (AOS): vitamin C (Vit C) i vitamin E (Vit E) su značajno smanjene u jetri kod pacova tretiranih kadmijumom. Eksperimenti sa pacovima koji su u pred tretmanu dobijali maslinovo ulje pokazuju da maslinovo ulje sprečava ili ublažava kadmijumom izazvane promene u koncentraciji LP, aktivnosti, kao i koncentraciji endogenih antioksidacionih zaštitnih komponenti