Ethnopharmacological relevance: Ganoderma lucidum (W. Curt:Fr.) P.
Karst. has been used for several thousand years in traditional medicine
of the Far East for the treatment of a various diseases including:
cancer, immunological disorders, hepatitis, arthritis, nephritis,
bronchitis, asthma etc. Ganoderma species are natural sources of various
antimicrobial, antioxidant and antitumor active compounds which can be
alternative to commercial antibiotics, antimycotics, antioxidants and
cytostatics. As the amount of wild Ganoderma lucidum basidiocarps is not
sufficient, the aim of this study was to compare the bioactivity of
basidiaocarps obtained on alternative substrate (wheat straw) with
commercially cultivated ones.
Materials and methods: Antimicrobial potential of the tested Ganoderma
lucidum extracts was studied by disc-diffusion and microdilution method.
Cytotoxic effect of tested extracts on two cancer cell lines was
determined by microculture tetrazolium test. DPPH scavenging ability,
total phenols and flavonoids content were measured in order to determine
the antioxidative potential of Ganoderma lucidum extracts.
Results: Ganoderma lucidum BEOFB 434 extract was the most effective
antibacterial agent, while commercial strain was the weakest. DPPH
center dot scavenging activity of extracts of basidiocarps from
alternative substrate was also significantly higher than that of
commercially obtained basidiocarps. The extracts of Ganoderma lucidum
BEOFB 431 exhibited the strongest cytotoxic activity significantly
higher than those in commercial strain.
Conclusion: The study showed that fruiting bodies produced on one of the
most accessible and cheapest crop residue, wheat straw, are better
antimicrobial, antioxidant and cytotoxic agents than those obtained on
conventional substrate, oak sawdust. (C) 2014 Elsevier Ireland Ltd. All
rights reserved.Ministry of Education, Science and Technological Development of Republic
of Serbia {[}173032