This study aimed to assess the anthropogenic activities at Rio Doce Park, Minas Gerais, Brazil, from ethnopharmacological surveys in Timóteo and Marliéria, which are located around the park. Interviews were conducted with previously identified, key informants, 15 in Timóteo and 10 in Marliéria. Two respondents collected medicinal plants in the forest of the park (from a few trees) but kept the same vulnerability of the use of their barks. Among the 141 surveyed botanical terms, we identified 95 species of 44 different botanical families. On the basis of statistical analyses, the 12 most used species were selected by respondents considering their therapeutic purposes and also obtaining the purpose of the use and dosage, among others. The knowledge about the use of medicinal plants has been maintained through generations but away from the formal health system and a sustainable management plan to encourage the preservation of the park
