Glede na prgišče člankov objavljenih objavljenih pred štiridesetimi leti, morska vetrnica Metridium senile vsebuje citolitičen protein, ki ga inhibira holesterol. Prav tako naj bi citolitične proteine vsebovala morska vetrnica Alicia mirabilis, njihovo prisotnost nakazujejo medicinska poročila o poškodbah ob kontaktu z njo in nedavna diplomska naloga. Za oba proteina smo predvidevali, da spadata v naddružino MACPF/CDC. V magistrski nalogi smo uspeli delno izolirati in očistiti oba proteina in določili nekatere njune biofizikalne lastnosti. Oba proteina sta velika okoli 55 kDa, temperaturno labilna z Tm pri 65 °C. Optimalna pH vrednost hemolize je 5,5 za Metridium oziroma 7,0 za Alicio, medtem ko je aktivnost obeh inhibirana pri pH 8,0. S testi vezave na umetne lipidne vezikle nismo ugotovili velike specifičnosti za posamezne lipide. Pridobili smo tudi aminokislinski sekvenci N-konca obeh proteinov in cDNA sekvenco citolitičnega proteina iz M. senile.According to a handful of papers published more than forty years ago, sea anemone Metridium senile contains cytolytic protein that is inhibited by cholesterol. Similarly, sea anemone Alicia mirabilis contains cytolytic protein which is probably the cause of several stings upon the contact with the anemone, as described in several medical reports. Both proteins are putative members of MACPF/CDC superfamily. In our research, we managed to partly purify both proteins and to determine some of their biophysical characteristics. Both proteins have molecular masses of approximately 55 kDa and are thermally labile with Tm at 65 °C. Optimal pH values for haemolysis are 5,5 (Metridium) and 7,0 (Alicia) while pH 8,0 proved to be inhibitory for both. Experiments with artificial lipid vesicles did not show strong biding specificity for used lipids. Additionally, we acquired N-terminal amino acid sequence for both proteins and cDNA sequence of cytolytic protein from M. senile
