ghaseminejad, Atosa

Simorgh Research Repository

 I    ﺧﻼﺻﻪ ﻓﺎرﺳﻲ  ﻛﺮﻣﺎن درﻣﺎﻧﻲ ﺑﻬﺪاﺷﺘﻲ ﺧﺪﻣﺎت و ﭘﺰﺷﻜﻲ ﻋﻠﻮم داﻧﺸﮕﺎه  داروﻳﻲ ﻋﻠﻮم و داروﺳﺎزي داﻧﺸﻜﺪه   داروﺳﺎزي ﻋﻤﻮﻣﻲ دﻛﺘﺮاي ﻧﺎﻣﻪ ﭘﺎﻳﺎن   :ﻋﻨﻮاندﻳﻜﻠﻮﻓﻨﺎك ﺳﺪﻳﻢ  ر ﺳﻤﻴﺖ ﻛﻠﻴﻮي اﻟﻘﺎ ﺷﺪه ﺗﻮﺳﻂاﻧﺪاﻧﺴﺘﺮون د ﻣﻄﺎﻟﻌﻪ اﺛﺮ  در ﻣﻮش ﺳﻮري ﻧﺮ    :ﺗﻮﺳﻂ  آﺗﻮﺳﺎ ﻗﺎﺳﻤﻲ ﻧﮋاد  اﺳﺎﺗﻴﺪ راﻫﻨﻤﺎ:  رﺿﺎ رﺣﻴﻤﻲ ﺣﻤﻴﺪ دﻛﺘﺮ دﻛﺘﺮ اﻟﻬﺎم ﺟﻌﻔﺮي  دﻛﺘﺮ ﻣﺠﺘﺒﻲ ﺷﻜﻴﺒﺎﺋﻲ    اﺳﺘﺎد ﻣﺸﺎور:  دﻛﺘﺮ ﺣﻤﻴﺪ ﻓﺮوﺗﻦ ﻓﺮ   4301ﺷﻤﺎره ﭘﺎﻳﺎن ﻧﺎﻣﻪ:                                                                   7931ﭘﺎﻳﻴﺰﻲﺳرﺎﻓ ﻪﺻﻼﺧ    II  Kerman University of Medical Sciences Faculty of Pharmacy   Pharm. D Thesis  Title: Investigation of the effect of ondansetron on diclofenac sodium-induced nephrotoxicity in male mice  By: Atoosa Ghasemi Nejad  Supervisors: Dr. Hamid Reza Rahimi Dr. Elham Jafari Dr. Mojtaba Shakibaei    Advisor: Dr. Hamid Forootanfar  Autumn 1397                                                                         ThesisNo:1034  III    ﺧﻼﺻﻪ ﻓﺎرﺳﻲ   ﺧﻼﺻﻪ ﻓﺎرﺳﻲاز ﺣﻴﺚ ﻛﺎﻫﺶ اﺛﺮات درﻣﺎﻧﻲ ﻳﺎ اﻓﺰاﻳﺶ ﻋﻮارض ﻏﻴﺮ ﻣﻨﺘﻈﺮه داروﻫﺎ ﺑﺎﻳﺪ ﻫﺎي داروﻳﻲ  واﻛﻨﺶ ﻣﻘﺪﻣﻪ:اﻟﺘﻬﺎﺑﻲ ﺗﻬﺪﻳﺪ ﻛﻨﻨﺪه زﻧﺪﮔﻲ از داروﻫﺎي ﺿﺪﻣﻮرد ﺗﻮﺟﻪ ﺑﻴﺸﺘﺮي ﻗﺮار ﮔﻴﺮد. ﺳﻤﻴﺖ ﻛﻠﻴﻮي ﻳﻚ ﻋﺎرﺿﻪ د و اﻟﺘﻬﺎب ﻣﻮرد ﺑﻪ ﻃﻮر ﮔﺴﺘﺮده در درﻣﺎن در اﻳﻦ دارو ﺑﺎﺷﺪ ﻛﻪ ﻴﺪي ﻣﺎﻧﻨﺪ دﻳﻜﻠﻮﻓﻨﺎك ﻣﻲاﺳﺘﺮوﺋﻴﺮﻏﮔﻴﺮﻧﺪه ﺳﺮﺗﻮﻧﻴﻦ از ﻗﺒﻴﻞ  ﻫﺎي آﻧﺘﺎﮔﻮﻧﻴﺴﺖﺑﻪ ﻧﻘﺶ ﻣﺤﺎﻓﻈﺘﻲ ﺎت ﮔﻴﺮد. در ﻣﻄﺎﻟﻌ اﺳﺘﻔﺎده ﻗﺮار ﻣﻲ ﺑﻨﺎﺑﺮاﻳﻦ در اﻳﻦ ﻣﻄﺎﻟﻌﻪ اﺛﺮ اﺷﺎره ﺷﺪه اﺳﺖ. ﻫﺎي ﻣﺨﺘﻠﻒ از ﺟﻤﻠﻪ ﺳﻴﺴﺘﻢ ﻋﺼﺒﻲ اﻧﺪاﻧﺴﺘﺮون ﺑﺮ ﺑﺎﻓﺖ ﻣﻮرد ﺑﺮرﺳﻲ ﻗﺮار ﮔﺮﻓﺖ.در ﺳﻤﻴﺖ ﻛﻠﻴﻮي ﻧﺎﺷﻲ از دﻳﻜﻠﻮﻓﻨﺎك  ﺗﺠﻮﻳﺰ اﻧﺪاﻧﺴﺘﺮونه ﺗﻘﺴﻴﻢ ﺷﺪﻧﺪ: ﮔﺮوه ﮔﺮو 5ﺳﺮ ﻣﻮش ﺳﻮري ﻧﺮ ﺑﻪ ﺻﻮرت ﺗﺼﺎدﻓﻲ در  03در اﻳﻦ ﻣﻄﺎﻟﻌﻪ  روش ﻛﺎر:دﻳﻜﻠﻮﻓﻨﺎك ﺳﺪﻳﻢ ﺑﻪ ﮔﺮم ﺑﺮ ﻛﻴﻠﻮﮔﺮم ﻣﻴﻠﻲ 001دوز  ﮔﺮوه دوم ه ﻛﻨﺘﺮل آب ﻣﻘﻄﺮ،ﺑﻪ ﻋﻨﻮان ﮔﺮو اولﮔﺮم ﺑﺮ ﻣﻴﻠﻲ 1و  0/5، 0/1 ﻫﺎي ﻣﺨﺘﻠﻒ دوز ﻫﺎي ﺳﻮم ﺗﺎ ﭘﻨﺠﻢﮔﺮوه و ﺧﻞ ﻋﻀﻼﻧﻲﺗﺰرﻳﻖ دا ﺻﻮرت روز 3 در و ﺗﻴﻤﺎر روز 7ﻣﺪت  ﻃﻮل در ﺗﺰرﻳﻖ داﺧﻞ ﺻﻔﺎﻗﻲ ﺑﻪ ﺻﻮرت داروي اﻧﺪاﻧﺴﺘﺮون را ﻛﻴﻠﻮﮔﺮمﺪ ﺳﺎﻋﺖ ﺑﻌ 42درﻳﺎﻓﺖ ﻛﺮدﻧﺪ.  دﻳﻜﻠﻮﻓﻨﺎك ﺳﺪﻳﻢﮔﺮم ﺑﺮ ﻛﻴﻠﻮﮔﺮم ﻣﻴﻠﻲ 001ﻫﻤﺮاه ﺑﺎ  اﻧﺪاﻧﺴﺘﺮون آﺧﺮﻜﺮد ﻛﻠﻴﻪ ﻠﻫﺎي ﻋﻤﺺﺷﺎﺧدوز درﻣﺎﻧﻲ ﻧﻤﻮﻧﻪ ﺧﻮن ﺣﻴﻮاﻧﺎت ﺟﻤﻊ آوري ﮔﺮدﻳﺪ. ﺳﭙﺲ  از آﺧﺮﻳﻦﮔﻴﺮي ﻓﻌﺎﻟﻴﺖ  ﺪازهﺟﻬﺖ اﻧ ،ﻛﻠﻴﻪ ﻧﻤﻮﻧﻪ ﺑﺎﻓﺖﮔﻴﺮي ﺷﺪ. اﻧﺪازهاوره و ﻛﺮاﺗﻴﻨﻴﻦ ﺳﺮم ﺧﻮن ﻏﻠﻈﺖ ﺷﺎﻣﻞ  ﺑﺮرﺳﻲ ﻫﻴﺴﺘﻮﭘﺎﺗﻮﻟﻮژيو  ﻛﻠﻴﻮي اﻛﺴﻴﺪ دﻳﺴﻤﻮﺗﺎزﺳﻮﭘﺮﮔﻠﻮﺗﺎﺗﻴﻮن،  ﻣﺎﻟﻮن دي آﻟﺪﺋﻴﺪ، آﻧﺰﻳﻢ ﻛﺎﺗﺎﻻز، . در ﻧﻬﺎﻳﺖ اﻃﻼﻋﺎت ﺟﻤﻊ آوري، ﻣﻘﺎﻳﺴﻪ و ﻣﻮرد آﻧﺎﻟﻴﺰ ﻗﺮار داده ﺷﺪﻧﺪ.ﺘﻪ ﺷﺪﺑﺮداﺷ VI    ﺧﻼﺻﻪ ﻓﺎرﺳﻲﻠﻮﻓﻨﺎك ﺳﺪﻳﻢ ﻣﻨﺠﺮ ﺑﻪ ﻛﻪ ﺗﻴﻤﺎر ﻫﻢ زﻣﺎن دو داروي اﻧﺪاﻧﺴﺘﺮون و دﻳﻜ ﻣﻄﺎﻟﻌﻪ ﺣﺎﺿﺮ ﻧﺸﺎن داد :ﻧﺘﺎﻳﺞ. ﻣﻘﺎدﻳﺮ ﺳﺮﻣﻲ ﺷﻮد ﻣﻲ ﻫﺎي ﻋﻤﻠﻜﺮد ﻛﻠﻴﻪ ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﻛﻨﺘﺮل ﻣﻌﻨﺎداري در ﻫﻤﻪ ﺷﺎﺧﺺ اﺧﺘﻼفره ﮔﺮوه ﻫﺎي درﻳﺎﻓﺖ ﻛﻨﻨﺪه داروﻫﺎي اﻧﺪاﻧﺴﺘﺮون ﺑﻪ ﻫﻤﺮاه دﻳﻜﻠﻮﻓﻨﺎك ﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﻛﺮاﺗﻨﻴﻦ و اون اﻧﺪاﻧﺴﺘﺮون و دﻳﻜﻠﻮﻓﻨﺎك ﺑﻪ . در ﺗﻴﻤﺎر ﻫﻤﺰﻣﺎﻧﺸﺎن دادرا  (˂P0/100)ﻛﻨﺘﺮل اﻓﺰاﻳﺶ ﻣﻌﻨﺎداري  ﺳﻮﭘﺮاﻛﺴﻴﺪ دﻳﺴﻤﻮﺗﺎز ﻫﺎي ، ﻛﺎﻫﺶ ﻓﻌﺎﻟﻴﺖ آﻧﺰﻳﻢP(˂0/100) ﺪﻫﺎ، اﻓﺰاﻳﺶ ﻣﺎﻟﻮن دي آﻟﺪﺋﻴ ﻣﻮشﻧﺴﺒﺖ ﺑﻪ ﮔﺮوه ﻛﻨﺘﺮل ﻣﺸﺎﻫﺪه  ˂P(0/100)ﮔﻠﻮﺗﺎﺗﻴﻮن ﻣﺤﺘﻮاي و  ˂P(0/100)ز ﻻﻛﺎﺗﺎ ،(P<0/100)ﻫﺎ، دژﻧﺮاﺳﻴﻮن  ﺧﻔﻴﻒ ﮔﻠﻮﻣﺮول ، دﻳﻜﻠﻮﻓﻨﺎك ﺑﺎﻋﺚ آﺗﺮوﻓﻲﻫﻴﺴﺘﻮﭘﺎﺗﻮﻟﻮژيدر ﺑﺮرﺳﻲ  ﻫﻤﭽﻨﻴﻦ ﺷﺪ.ﺷﻮد  ﻫﺎ ﻣﻲ ﺗﻮﺑﻮل ﻟﻮﻣﻦ در ﺻﻮرﺗﻲ رﻧﮓ ﻫﺎ ﻫﻤﺮاه ﺑﺎ اﺗﺴﺎع و ﺗﺠﻤﻊ ﺗﺮﺷﺤﺎت ﺗﻮﺑﻮل ﺗﻠﻴﺎلﻫﺎي اﭘﻲ ﺳﻠﻮلﻫﺎ و ارﺗﺸﺎح ﺗﻠﻴﺎل ﺗﻮﺑﻮل ﻫﺎي اﭘﻲ ﺳﻠﻮل ﻣﻴﺰان دژﻧﺮاﺳﻴﻮن ﺴﺘﺮون ﺑﺎ دﻳﻜﻠﻮﻓﻨﺎكاﻧﺪاﻧ ﺰرﻳﻖ ﻫﻤﺰﻣﺎنﻛﻪ ﺑﺎ ﺗ  ﺷﻮد. ﻣﻲ ﺑﻴﺸﺘﺮﻓﻀﺎي ﺑﻴﻨﺎﺑﻴﻨﻲ ﻫﺎي اﻟﺘﻬﺎﺑﻲ در  ﺳﻠﻮلﻛﻠﻴﻮي ﻧﺎﺷﻲ اﻧﺪاﻧﺴﺘﺮون ﺑﺎﻋﺚ اﻓﺰاﻳﺶ ﺳﻤﻴﺖ  ﺣﺎﺿﺮ ﺣﺎﻛﻲ از آن اﺳﺖ ﻛﻪﻧﺘﺎﻳﺞ ﻣﻄﺎﻟﻌﻪ  ﻧﺘﻴﺠﻪ ﮔﻴﺮي:  .ﺷﻮد از دﻳﻜﻠﻮﻓﻨﺎك ﺳﺪﻳﻢ ﻣﻲ  .ﺳﻤﻴﺖ ﻛﻠﻴﻮي، دﻳﻜﻠﻮﻓﻨﺎك ﺳﺪﻳﻢ، اﻧﺪاﻧﺴﺘﺮون و اﺳﺘﺮس اﻛﺴﻴﺪاﺗﻴﻮ ﻛﻠﻴﺪي:ﻛﻠﻤﺎت ﻲﺳرﺎﻓ ﻪﺻﻼﺧ    5 Abstract  Introduction: Drug-drug interactions require more consideration that reduced the desired effects in patients or increased the risk of unexpected side effects of the drugs. Nephrotoxicity is a life-threatening complication of nonsteroidal anti-inflammatory drugs (NSAIDs) such as diclofenac (DIC) that widely used as pain relief and have investigated to cause multiple organs damage. Serotonin 5-HT3 receptor antagonists such as ondansetron (ODT) have reported to exhibit protective properties in tissues including control nerve system in studies. Therefore, this study was designed to evaluate the effect of ODT on DIC induced nephrotoxicity in mice. Material and method: In this study, 30 male mice were randomly divided into five groups: the first group as control group received distilled water, the second group received DIC (100mg/kg, i.m.), the third to fifth groups received different doses ODT (0/1, 0/5 and 1mg/kg, i.p.) for 7 days. Twenty-four hours after the last dose, blood samples were collected from animals. Then, kidney function indexes including serum urea and creatinine concentrations were measured. Kidney samples were taken to measure the activity of catalase, malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and histopathologic studies.  Result: Studies have shown that simultaneous administration of both ODT and DIC leads to significant impairment in all kidney function indices compared to the control group. In the simultaneous treatment of ODT and DIC in mice revealed a significant increase in urea and creatinine levels and MDA concentration and marked reduction in catalase activity and reduced GSH concentration and SOD activity in compared with the control. In toxicological studies, mortality was observed when DIC and ODT were given to mice, but in DIC alone, mortality was not observed in mice. Histopathologically, DIC produced atrophic glomeruli and eosinophilic casts were detected in the renal tubules those were exacerbated by administration of ODT prior diclofenac.  Conclusion: The results of this study indicate that ODT increases the renal toxicity of DIC. Keyword: Renal toxicity, Diclofenac sodium, Ondansetron, Oxidative stress  6    ﺧﻼﺻﻪ ﻓﺎرﺳﻲ 

مطالعه اثر اندانسترون در سمیت کلیوی القا شده توسط دیکلوفناک سدیم در موش سوری نر

http://eprints.kmu.ac.ir/29888/1/6943.pdf

مطالعه اثر اندانسترون در سمیت کلیوی القا شده توسط دیکلوفناک سدیم در موش سوری نر

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