Neural differentiation of human umbilical cord matrixderived
mesenchymal cells under special culture conditions
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Abstract
Many neural disorders are characterized
by the loss of one or several types of neural cells.
Human umbilical cord-derived mesenchymal cells
(hUCMs) are capable of differentiating into neuron,
astroglia-like and oligodendrocyte cell types. However,
a reliable means of inducing the selective
differentiation of hUCMs into neural cells in vitro
has not yet been established. For induction of neural
differentiation, hUCMs were seeded onto sterile glass
slides and six various cocktails using a base medium
(DMEM/LG) supplemented with 10 % FBS, retinoic
acid (RA), dimethyl sulfoxide (DMSO), epidermal
growth factor (EGF) and fibroblast growth factor
(FGF) were used to compare their effect on neuronal,
astrocyte and oligodandrocyte differentiation. The
hUCMs were positive for mesenchymal markers,
while they were negative for hematopoietic markers.
Differentiation to adipogenic and osteogenic lineage
was detected in these cells. Our data revealed that the
cocktail consisting ofDMEM/LG, FBS, RA, FGF, and
EGF (DF/R/Fg/E group) induced hUCM cells to
express the highest percentage of nestin, ß-tubulin
III, neurofilament, and CNPase. The DF/Ds/Fg/E
group led to the highest percentage of GFAP expression.
While the expression levels of NF, GFAP, and
CNPase were the lowest in the DF group. The least
percentage of nestin and ß-tubulin III expression was
observed in the DF/Ds group. We may conclude that
FGF and EGF are important inducers for differentiation
of hUCMs into neuron, astrocyte and oligodendrocyte.
RA can induce hUCMs to differentiate into
neuron and oligodendrocyte while for astrocyte
differentiation DMSO had a pivotal role