Layegh Khavidaki, Fatemeh

Simorgh Research Repository

ltP,t,,{,F* 6,(,i.'.13.,S';,-= .r*1-,i.1-oJs*. .l;Jl J-t'.,}.JL< ^ti- a-"t;L!:;l;,-,c(ica) at o3SsJ 3 Immune-Evasion-Cluster (IEC) ,_5tj csLag aE:-,,;+jlS .gLa<-,3,-, ;l o.ulli .,*,:rt:t .sL^.S-r<-rl*ti.,.l ;.r Intercellular adhesl -'::;lr,,l ,;LJ J.6-;..-5+s= ;Y <-JG o 'j', ,L' '=f.ri+;r.-:l !r: :Kr-Ff,,.:, ,::)t< .r3l.r ;iS.r :L&l; :1.I--'\Yl o-\ Y11 :,rl*^-; Jt*,Kerman University of Medical SciencesFaculty of MedicineLr Paftial Fulfilln-rent of the Requirements for the Degree of Master of Science(MSc)Title::Determination of Immune-Evasion-Cluster (IEC) gene fypes andlntercellular adhesion (ica'S in clinical isolates of staphyloccus oureuscollected from Kerman, IranBy:Seyyedeh Fatemeh Layegh KhavidakiSupervisors:1- Davood Kalantar Nistanaki (Ph.D)2- Roya Ahmad Rajabi (Ph.D)Year:2017:si-i->)?;>,Gi;1-;i c*1;- crr<Jl ,4J1t g,-l 1r.-r5,r. U,-l l) Lr-+" "S..r-Y:,p_9:iSlSt 9 4oJi.c-,Sf l*rlL"l r: (tEC),*;_l-"-*- ) )f 6r;Xf ty s l*xrJr,!,:1 t[,,lr icaABCD ,-ri _*_- _chp 6t-a$ crgl- IEC ufi +9^><^..:,*l or; ;y; uly-l ,O\rt Ai J+lt .rlba;]^i;l o-r; I.r> c]i.i - -"r.l5rl) sak ,(6tJ.5 or51\3 eldptr 6r;5rf) scn ,(uSs:e.i ori)l{d S6Es.t :-_-_-'_,ti)." *:--l o;-*- ,,l4f ,; al r;[,r. P Or-t y *l "r::frt) sep , (A a_*S i:yl ori-rf ) sea ,(]*j -rJE c*a ,i-"if ,SJrl-N .5lo"sl9 !.5-r,_1llL,r!, oyt;'l)12.-l J+- icaABCD G; ,:"f | .*.: _',l.,tr jl ,:"i:l .Sfrrk-l *<rJtale;-l \.. ,(/€;*;a."-:rl ti'AA,ti )"tV J+ 1;:tb9.i91 9: -;.* i9lqr.(---r;9, L-i ,jqyr;i ;*rU;.ri.5,19i6^' uvf ,-r.)l.l .'5lorb,;Lqr ar :i+ -O&.r- ! f9la" .51-oal9;-l .r.L|;; I J}x !n c.t- laaJ9;l .4Llr, ;*.; el.t u*ys ;;*._-_..5laal9;l ,-"_9^ a;.i)ruc 6l-a;) oai-; cap (PCn) ;1,**t- .51or.2") ,5lau;519.$ i,,>..9 (sep t:ieo .chp ,sak ,scn )lEcp)af cs6..rj ,(MRsAd[+l9r1l ,r,-LL;\nec ,(u,;,i' r ,.$ r$l icaAB( Lt -u. Lyr (-r-r: 2AlY) +;l.; Y. ,:1u ;,-l jl r; .,,-Ltji MRSA aJr;l ff .4j9- \.. ,lr=" :' :b;: -a, l-oaJ9;_l a-o L e,_U ,,) .r9, CA-I\{RSA ;111"* + Lyr (-r-r: l\/A) dj}.j \f 9 H-{-\Ii . -,r**-Ll-riJl ,o?,L-S9l3ty*,;*-lS.,i .Slo.!t.r-i + *9t-i. .ri:2 ,,Lo J_Yi,.= , _ror: YA 9 Af ,YY ,AY ,f \ ,Y1 .YA !-*," t Jy6!4y"-e.eid-i 9 ;*K--Llr: (gr^*-: -iMSSA.5loaJerll ;l (0/ap) I ttlt, MRSA.5traJe;_t ,l (yf/ff) /.vvlt af rl:;L; r ::_ _;-)K .S6.tj ;lrlr-i .-r-; oroL;o /. ltt ;lp + ;**_Ll-r:Ji .r1l,iJl c-91,i. .u;1: (\{DR. _ . -),;-)4-l )y;> ); or,rL;- ly l,sep 6 s/.\. L sea ,lyaL.chp, /.af L scn ,'y.?. _ .,->ll e_L; "* .(P:0.000) :y I\,IRSA .5toal9;,_1 ) fu.5rbL* ,et a, N{SS-\ :-:._. _.,oJ*s* .5lrlr a;r^; fA , L";- ol+ry.5l1l: uo\p ,Sf l-,:ryy..glrl: a,9", i ,L 6,_; .: .:. - *r..'/ { icaC gicaB,icaA 6la;).r;;&it_ 1^u::^, )ltS ,>icaD;).i)- }1..= _i. _-_.JJ;; +-,;AbstractIntroduction:Resistance to antibiotics and presence of virulence factors play an impoftantrole in increased mortality associated with infection due to Staphl;locot:clts all'eus.ln this study,u,e detennine antibiotic resistance pattern, presence of tlie ica,4DBC locus as well as biofilmformation and distribution and diversity the immune evasion cluster (IEC) genes in clinicalisolate of S. aureus from Kerman. Iran. The tEC encodes the immune-modulating proteins suchas chemotaxis inhibitory protein (CHIPS). staphylococcal cornplement inhibitor (SCIN).staphylococcal enterotoxir.r A (sea), staphllococcal enterotoxin p (sep) and staphylokinase(sak). Tlre icaADBC locus encodes genes lead to the biosl'nthesis of poly'saccharide intercellularadhesion (PIA) rnolecules u'hicl, is composed of N-acetylglucosamin to biofilm formation.N{aterial and methods:During 7 months. 100 clinical isolates S. uureus recovered fromdifferent patier,ts were adrnitted to Kerman UrTiversity affiliated hospitals. Resistance todifferent antibiotic agents u'as determined by disk diffusion method. Phenoty'pic method wasuSed to determir,ation of biofilr-n tbrnration ability and methicillin-resistance S. atu'eus (MRSA).Poll,nrerase chain reaction tecl,nique (PCR) rvas used to detection of nuc'll.dentificatiorr ofStaphl'lococcus aureus isolates), mec'A(recognition of N4RSA), the ic'uADBC locus and The IECgenes(^scr, sea, suk, sep and c'hb).Results:For11'-four isolates u,ere considered as N4RSA (68.2% isolates HA-MRSA and31.8% CA-MRSA). All of isolates n'ere sensitive to vancoml'cin and linezolid. Resistance toantibiotics amikacin. ciprofloxacin, clindaml,cin. erl,throrrycin. gentamicin and tetracyclineand trinTethoprirr/sullamethorazole \\,as 25.39.31.52.33.5.1. and 38% respectively. Ourresults shou'ed. 77.2% (31111) of MRSA and 8.9 %( 5156) of N,ISSA isolates rvere rnr:ltidrugresistant. Inducible clindarnycin resistance rate was l8o/o. cltp \\'as presenl in 35oh of thesestrains. .s('r7 was in 51o,/o. .rak u,as in 60n/o. .sea and -rep \\ere in 109/o and 35% respectively. Therervas significant diff'erence in presence of IEC tlpes betu,een N4SSA and MRSA isolates(P:0.000). The predomiuant IEC variant rvas tlpe B. The ability to production biofilm in9(9%)isolates rvas strong. 26 (26%) isolates u'as moderate..lS (.18%) isolates u,as u'eak and 17(17%)of therr lrad no production biofllm. The prevalence of icctA, icctB. icctL- and icctD in all of isolatesn'as 270. 1%^ 2% and 81o/o respectively'. There \\'as no significant difference in productionbiofilm betu'een l\4SSA and MRSA isolates and No sienillcant lelalionship n'as lound betileenthe it't-lBCD locus arid biofilrr fbrmatiort (P>0.05).Conclusion:Antibiotic resistance in clir-rical samples of lterman. espt'cially in \{RSA-itririns is ii:creasirrg. The presence of the icoADBC locus i-nay not be deterrrining lactor forbiof-ilm fonration. our data ma)' support some published data based on that biofilrri fomiationnray rely ort enviror-rnientally regulated. ic,aADBC-in dependent mechanisrn (s). IEC has spreadluccessfully through lhe S. au"eus population and will continue to do so. This enable s S. aurens.-' ith a unique mechanism to adapt to. and counteract, the hurnan host.Keyr,r'ords: Staphylicocclts oureus. Antibiotic resistance. ica.4BCD locus. Biofi]m. IEC:\ pesf\tLrl a.*ra,U 6tr-L, 11 6G; d*la-t'ryo€_-)l: '\t:,. -*' 9!-, ,b .' lii prlj , -ol-(:il: IcC, - --,(-y"*(:r,#f .dLi./g,F$ ,::)r))l.r-:K sF> J*-f-3,l"I 4^1,t9o.\-FI[>.i}-a;&ulL-L;'"r'6:*-.Ligx,i )lo*! 1"r> dfrldrts e.fgflGU-Jf ;,r (ica ) "lj..ffd 9 (lEC ) .lj,"gtA.'^.' O+." " jlrr c-- au'f ,*t t-'t" - *:;t _f5*:*p1;11:+L^ , r*rgl;s'\_'r*1. 10/ ll!'/ r?_*qi,i&j-r-, 1.*-pLr:"61yt.ut{f xi,*fO{ *t'La^llJslu6 pU q pUg / | 1l *..&,.,.$U+ y),Lf cglc rt's -<tlI ";Li>.ro; "r+>l b-l; rfJ ett ttflL-.,tI; (;l):.,;-1:Jl{}-d ;1Li >L:-i :-, c ro,al* >loi 5s *iti rtf *,9J)----'S-- ;- J )-ffif* , clro.c ;ljl :,-rT . '.61 -t* 5l -cGT *.rL;,->97_'7'u< - - v- (-a;*) 91;11: *'La ,-ri^['-tt{,'t^,_../iJu;fiitwosl i -obte '; i .g , ctgT .JL>'9 v"r -

تعیین تیپ های ژنی Immune- Evasion- Cluster ( IEC ) و لوکوس ژنی ( ica ) Intercellular adhesion در استافیلوکوک های اورئوس جدا شده از نمونه های کلینیکی شهر کرمان، ایران

http://eprints.kmu.ac.ir/28583/1/6637.pdf

تعیین تیپ های ژنی Immune- Evasion- Cluster ( IEC ) و لوکوس ژنی ( ica ) Intercellular adhesion در استافیلوکوک های اورئوس جدا شده از نمونه های کلینیکی شهر کرمان، ایران

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