Foroghi Nematolahi, Masoud

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6L"Js ..,.lb,lJ ,.sfi|{+ crLoli J ,j+i pds ot<,iril.rd3tf.r iJJs S csit -r3h o.lS.''-iiil.r!#J ,rA!S g;Lag3h g1l4r6ii .lsr"g;jL*,S';lo,r-og.os, t'fJisJ q.t u!!,(CSmellia sinensisl-.) ;+-, Lth u#I ot? ;Jl ., c*".., .,.'.,J313T3 g HT-zg;gJ*., g;La oo.; cggl ',;;:Ja-rg3,s{Xl ct6r-r ,*Sls .rJn-r.uo6.lJ+€ 4-JLaJ.S, irt- .lK.l-,Ct*J4a lJsf JjsJ ilB _1ts.1*ci;*ls,:f I -.3! ;-rsr $t5l :tr+\ftv oLo;y tlY :caLr ''t-rt-r .: .'.-r-Y-iJKerman University of Medical SciencesFaculty of PharmacyHerbal and Traditional medicines Research CenterPharm. D Thesis'Title: :' 'Evaluation of.cytotoxic activity of aqueous extract of Camellia sinensisL-on HT-29 and 3T3 cell linesMasoud Foroughi Ne'matollahiSupervisors:Dr. Salehe SabouriDr. Mitra MehrabaniDr. Bagher Amir-HeidariThesis No: 991Julv 2018u-1li a-c)o4-P>V)F r lJ i[-i.i- jl .E-,L^t q-y ,=-l a; € 6G LSjt-: jl .r(-,JlJ^, ! Jl!,-;L;: :+,-tj:,J-/- ut, o); -t; ,S:s G;V,;l .gl;l: .,lF;L^ o:;1iy.;.<\f ,-l -:.*l o:r5;*r71,.3l oiLrr.S-,\i- jl -.;. p-l ;: .i*l o:l:.rzL=:-l ,3- ! lr;LJL";1 .gl "-r..-c e+- oU,*JLS-,!_f Ur+-rlS;.:i) HT-29 ,J,1- "r, j) Gr) ; (Canellia sinensis L.) ):- dq ,=:.--"f 1l; +Jlt -:;y 31'3 J, ( :--<ol- ro ! JIL \. . .. :l-t-r .DMEM t* ;: "-u; ;: .!yL "r, y: -^5;1 , * :Ls ;rJ--*?- .-r .l:.*!:-(l .:;L- YY J-1. ! .l: CO: ;--!;r(l ;) j -u ":3.j1 a-iE Ct l+-_.-_!.J:l-" r (; *+;, 1j-,,S* '/'t-t "'\ u-,:,-..;y, "Lj ti "'L-o rl "-t-; +^a,..<l--: -.^;"-t; ,5! iL, ;LrL- ;,: ; -L; ;t--l ,-(-: ;pL YA -o Yf .gl- -,, J) ;. o--"-!-i-(l 3 o-u o-sL; -..+rt' "r-L. JrL ,r, ,Q. l1;"" ; o-u; o:;;l -<"12 I a. MTT . ,_-...^. rl- .i ^.r -.- 1.1 ..- ^l-,(r.. -',1 ,: -a)) J J:- o= ,i o)G. Jjll- l:-"* C-rB a5 -Lr: .j" JL:j ,i--i-,--i -,,1 ,fl J-tr 5U :6-:L--r' L. J-*L ,Q. e-e ;*; _b -:, ! : o)-,t ,*-* ;-,1.,, a, 3T3 ,l FIT-29 J-.L ,:JL,; .5Lo Jl|- I c;-*: ;U,* e;L" J-+- tS:: j o;L."* oS *l ;i" j,-{ -,:l o,:--..-,;i -,:J-rL .r, 6lr'. ;AL ! e:l-r iJt- .i- )) r:- 6l' ,i "-L-; IC5s .:;l: S*. G-:=- _-VV/Yo+\./\\ J Yrq/AA+\0/VA y: r,i a-t 4--:rL- fA ; +-rL- Yf rL_-__;.1 _-; HT-_-.s+ r: f-5:r* 11Arf0*Yl'r1t ; \... ,l u;:.3T3 J:L "r, 6lt )r--J .,!. ;: ,,3.--<---j1G a-* -:l;r: _:.- -.q _i; o-La; .-i.--aa: '--t -: .-L,i +) 4r --Uj _-L*l -. :Sf o-il*1<:t +..,),iAbstractIntroduction: Treatrlent of malignancy as one of the most irnportant diseases in t,r-current century has beert aimed by rnany investigators. Natura products capable ..inhibiting cancer cell lines gror,vth has an impoftant role in this screeniug. The present srtr:i .rvas designed to evaluate tlte cytotoxic activity of aqueous extract of Canellia sinenstslon HT-29 (human colorectal adelrocarcinoma) and 3T3 cell lines.Methods: Cell lines rvere cultivated in DMEM. For cytoricity evaluatior.r, 10000 cells .,logarithrric phase r,vere seeded into each. u,ell of a 96-rvell microplate and incLrbatecl ,,37'C and 50 COzfor24 hoLrrs follorved by addition of prepared herbal extract (0.01-l rpg/mL) to each rvell. After 24 and 48 lroLrrs cell viability rvas determined using MT-f a-<..,.rrethod and the inhibitor.v effect of dach concentration of extract r.vas reportecl.Results: The results of this study shorv that aqueous extract of Canellia sinensis L. ',-,rnore cytotoric effects on HT-29 lhan iT3'cells. Consiclering the results of inhibitit:r -cells by doxorubicin, it rreans that the extract lras more inhibitory eflect on ctnceroLrs .:than normal cells. The stLrdies revealed the IC56 values of 239.88+15.78 pg/mL ancl ,. ..-1000 pg/rnl for24 hours and 71.25+10.12.pg/mL and 998.45+23.96 pglnl for l8 i.:. ,on the HT-2g and 3T3 cells respectively.Conclusion: Based on the results of this study, the aqueous extract of _green rc-rr ',,:significant cl,totoxicity ott colorectal cancer cells.Key words: cytotoxicitv, green tea, aqueous extract. HT-29. 3T3.gtof r'jh.,o@,lil,r+ erlotig o5ilfff" otliJlo.g.1L91lo cuAijblll :clhi o l. 6orrr.91L9.1ls .ggcuijls.oigj o9lm .gliT &li OQQ:drb,i, r;,rjt ttt - z e !/" gi t o t gf t t t ( canw f,fi a si n e n sf s L ) 1 ui b? ()i, rz lp u it ) t 6t u,,"ata/D\ / eU'*" l;.- rrr -YF :.19lio .rjt*l: hall .rfl*lcs;*:;*l.p! .,1*5" -1rl+ rlXa iSyy3-* t+-i F. -Yi).ef jb).ed i5r -f:Gll J99 !,t'j c{ gt.19lo pj*l *ip{q (5 . (r.rs. !) .+ \ e,-*sf )p,t1 t)),;

بررسی سمیت سلولی عصاره آبی چای سبز  ( Camellia sinensis L.) بر روی رده های سلولی  HT- 29 و 3T3

http://eprints.kmu.ac.ir/28411/1/6613_20180825_0001.pdf

بررسی سمیت سلولی عصاره آبی چای سبز ( Camellia sinensis L.) بر روی رده های سلولی HT- 29 و 3T3

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