Samie nezhad, Mohammad

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u1fr. rlt o?"(,i.',, o r(,i,'.t.1\,JI, -rl'..1. , ur o !, o d ,l . -rl'..1. 'L< ^t3- c-oL ,',LLlJvvr*Jv9JLv-.!:,-1lJi.)E-;lrl *,-1rl[S1 A: At** nlii- r B19 u,u]rrJ! q #rglT;l3lrs nif':\Y1o Jt*,-.1.1 ,ll-;S ,<*ii-.1.r f1*, tl.,pl ., +$.rJi. ..,G_r* !llj: ,rr.,^-., J-c:,-o : L. ,,$ '-o.ll-.1 ,-r;s. J.o='o .,Js, +f-, ;iS.l -;L:.el JJ* .SL tj; riS. :L&l; .lE*"|.,i)L [j3 .r.^-= ;i(.1 :;3Lii" .ll'1,,.1\Y11-\Y1V :,rl*,-..: Jt -,aKerman University of Medical SciencesFaculty of MedicineIIn Partial Fulfillment of the Requirements for the Degree MSc VirulogyTitle:_ Determination frequency of parvovirus 819 contamination and NSl geneexpression in patients with thyroid cancer and healthy subjects, KermanCity, 2016By:Mohammad Samie NezhadSupervisors:l-Dr Reza Malek Pour AfsharZ-Dr Seyed AIi Mohammad Arab ZadeAdvisor:Dr Hamid Reza MolaiYear: 2017-2018or'5?.c *l o$;.,-eelL o:lfL! "t 11J,;" s Fy,,J94,+f o"9y-e.i (B19) GGI ctx3erL :.il.ul9 4"aiool;;l 9 c*l .r^- Jl+)* J# aly ,:$y s,l .*l,V.S1 d,9r9 p$ A-i "r; !f csr*i;':,t jl .,.(1,ty"-\y)SX 4a, *, "Jj J+ J<r" C^-.- .r) r,*o' , *.x r'..-.4 .>rb ;U;, Ji- X.5l o-r5 ,=.-Pib+.l ,)-l.su .:rl: )*l Slx.rL.,- ,ib s ritl, c^iL riL kp!;l stV+. r: B19 tttN.9..),5..,J+g ,-s;\ lb..s,,y+,.! uV,- + )4.; ,lrL* ;l o+l,,t, S * orto rJlx .sta,i! jl o;i^, -r,-i- :l-r,; :b .r"9.rReal time 1l o.:1t-,,11 :1/S1 .-,j oL* 9Bl9 DNA .i-L-1;; c-a; \Y12 ii'\Y1Y .jt4JL ,a 1; 1Y9!9s.4rS )rt ,r";y rsr r-t\f F,r" p9.1c "t(;1:;: PCR.(Z tv; )rr c*i:. Bl9 o".t..erL DNA :U ) uy.'\Y ,4Jtt )tg. 49* \.. ;l al s.:l; ;L:J 6,-l; :Ll a;!11A/1 , Y \ ! rlr ,=4 y a- rjlx .rU,,- 4- yq- .rl,,L.: 1: AISI .rj .rQ ,L" .ilp.;l , OFL" j*o.-.n. :.,1-j:9>9 u;;l: .,;* o9l-0,' A1 ty I .l,-.rLX! Bl9 O"9y.99r!.tl:l,p ".1* oJit-;r-l ,::6.r*5 a-x;;"5r1.: ;* ;lG' u.*+ 9 ,;- i ril; rLr { ),1:.* ,lrk: ;", al rl: ;L;; .5rLi ;f-l-.; 9 il-.2- .-1*>.*}.:;1$:".',.1/S1 ,Real time PCR ,:is)r.rUr- ,Bl9 ,s"9y9erL :ojl9 .r{r)AbstractBackground and Objectives: Human parvovirus B19 (Bl9) is a small, non-enveloped virusand belongs to Parvoviridae family. The B19V genome contains a single promoter, p6, thatregulates expression of the major non-structural protein l/^S1. NS1 is cytotoxic to host cells819 persists in many tissues such as thyroid tissue and even thyroid cancer. The main aim ofthis study was to determine the presence of B19 and NS1 gene expression in patients withthyroid cancer.Method: 100 paraffin-embedded thyroid specimens of papillary, medullary and follicularcarcinomas were examined for B19 DNA and NS1 gene expression by Real time PCR, inKerman University of Medical Sciences.Result: The results have shown the presence of B19 DNA in 17 of 100 samples (%17). The- mean (SD) of //S1 gene expression in patients with thyroid cancer was 21 (8.9).' Conclusion: In this study there were no significant difference between parvovirus b19 withthe type "of cancer. Also, Statistical analysis showed there was no significant differencebetweeq thyroid cancer patients in age and gender.Key Words: Iy'S1, Parvovirus B19, Real time PCR, Thyroid Cancer14 4t. tr. 1 t'1. t'..,:!;i r;i r.ji'i,},1"1i.'.'.ai'tr;fun+x.J*- ***,' i! -i -rla'<u -'r.*, Jl f.t$J 4-*:lrJj*J-41*i;;31.,';Hxl.{lfiil.? idtet ,;?:*}id., ",,_J;'.qrPflifri;,"1i,r, J";,t ',.*e +, -it? ., -e--u!#d. {l l^ e'" -r,i'i t.YsrJ T+* * _S* *ard 3e * *ry. i;u;F- --!' .\ .1. ," Y i -i5, ,t -,).:"";Y_n*d s d ss r'*;re

تعیین فراوانی آلودگی به پاروویروس B۱۹ و میزان بیان ژن NS۱ در بیماران مبتلا به سرطان تیروئید و افراد سالم در شهر کرمان در سال ۱۳۹۵

http://eprints.kmu.ac.ir/28593/1/6643.pdf

تعیین فراوانی آلودگی به پاروویروس B۱۹ و میزان بیان ژن NS۱ در بیماران مبتلا به سرطان تیروئید و افراد سالم در شهر کرمان در سال ۱۳۹۵

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