A complex system for the production of pathogen-free grapevine propagating material

Abstract

The use of pathogen-free planting stock for new vineyard establishment is a key component in the maintenance and expansion ofvine and quality table grape production. The success of the necessary changes in the structure of the grape industry is forced by theglobalization process, the climate change, the rediscovery of autochton varieties as well as breeding of new tolerant and resistant varieties.The renewal of vineyards largely depend on the availability of planting stocks. Serbia and Hungary found a common interest in establishingpathogen-free stock materials from newly breed resistant varieties and clonal selections of varieties which are traditional in the Serbian-Hungarian border area. During a cross-border cooperation program a complex system for the production of pathogen-free grapevinepropagating material was established. Using heat therapy, in vitro shoot tip culture and traditional and molecular diagnostic techniques newpathogen-free stock materials were established from 26 varieties. They have been or will be tested for the presence of most importantgrapevine viruses, phytoplasmas, as well as bacterial and fungal pathogens. The complex system applying green grafting for indexing ongrapevine indicators can shorten the duration of the procedure from 4 years to two-three years

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