Hexavalent chrom (Cr(VI)) is recognized as toxic heavy metal, produced
from textile printing manufacture. Negatif effect of Cr(VI) shuold be reduced
towards biotic environtment, so it is important to reduce Cr(VI) become Cr(III)
which little hazard. Pseudomonas aeruginosa, Pseudomonas putida, Klebsiella
pneumonia, Pantoea sp and Saccharomyces cerevisieae. They are including as
resistent Cr(VI) microorganism and they have ability to reduce Cr(VI). The aim of
the research is to know the ability of microorganism in reducing Cr(VI) and
knowing protein band pattern between Cr(VI) resistent microorganism and
microorganism which is inoculated on LB broth without Cr(VI). Electrophoresis
SDS-PAGE is the methode which is used to indentified protein expression. While,
for knowing concentration Cr(VI) in liquid medium is used by 1,5 difenilkarbazid
methode. The quantitative data is obtained being analyzed by ANAVA two
factorial continued with Duncan test at 1% level test. The qualitative data include
protein expression analyzed by Relative mobility (Rf) and for knowing molecular
weight of protein is used protein marker. The qualitative data had been analyzed
by descriptive qualitative methode.
The result of the research shows that in Cr(VI) concentration after being
treat by the microorganisms at the initial concentration 0, 5ppm, 1 ppm, 5 ppm
and 10 ppm and compared the reduce Cr(VI) capability from the average
procentage data each microorganism are: P. putida (65%) > S. cerevisieae
(64,45%) > P. aeruginosa (60,73%) > Pantoea sp (50,22%) > K. pneumonia
(47,82%) > without microorganism (34,25%). The adding microorganisms have
influence toward reduction of Cr(VI).
Key word: Cr heavy metal, microorganism, protein, electrophoresi
